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Enhanced growth-driven stepwise inducible expression system development in haloalkaliphilic desulfurizing Thioalkalivibrio versutus
Sharshar, Moustafa Mohamed1,2; Samak, Nadia Abdrabo1,2,3; Hao, Xuemi1,2; Mu, Tingzhen1; Zhong, Wei1,2; Yang, Maohua1; Peh, Sumit1,2; Ambreen, Sadaf2,4; Xing, Jianmin1,2
2019-09-01
Source PublicationBIORESOURCE TECHNOLOGY
ISSN0960-8524
Volume288Pages:9
AbstractHighly toxic and flammable H2S gas has become an environmental threat. Because of its ability to efficiently remove H2S by oxidation, Thioalkalivibrio versutus is gaining more attention. Haloalkaliphilic autotrophs, like the bio-desulfurizing T. versutus, grow weakly. Weak growth makes any trial for developing potent genetic tools required for genetic engineering far from achieved. In this study, the fed-batch strategy improved T. versutus growth by 1.6 fold in maximal growth rate, 9-fold in 0.D-600 values and about 3-fold in biomass and protein productions. The strategy also increased the favorable desulfurization product, sulfur, by 2.7 fold in percent yield and 1.5-fold in diameter. A tight iron-inducible expression system for T. versutus was successfully developed. The system was derived from fed-batch cultivation coupled with new design, build, test and validate (DPTV) approach. The inducible system was validated by toxin expression. Fed-batch cultivation coupled with DPTV approach could be applied to other autotrophs.
KeywordThioalkalivibrio versutus Fed-batch cultivation Bio-desulfurization Inducible regulation system Promoter engineering
DOI10.1016/j.biortech.2019.121486
Language英语
WOS KeywordFED-BATCH CULTIVATION ; ESCHERICHIA-COLI ; SYNTHETIC BIOLOGY ; GENE-EXPRESSION ; PERFORMANCE ; VECTORS ; SULFIDE ; IRON ; TRANSLATION ; PROTEINS
Funding ProjectNational Science Foundation of China[21878307] ; National Science Foundation of China[31800030] ; National Science Foundation of China[31872633] ; CAS-TWAS (The World Academy of Sciences) President's Fellowship for International PhD students
WOS Research AreaAgriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS SubjectAgricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
Funding OrganizationNational Science Foundation of China ; CAS-TWAS (The World Academy of Sciences) President's Fellowship for International PhD students
WOS IDWOS:000472018100041
PublisherELSEVIER SCI LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/29956
Collection中国科学院过程工程研究所
Corresponding AuthorXing, Jianmin
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Egyptian Petr Res Inst, Proc Design & Dev Dept, Cairo 11727, Egypt
4.Chinese Acad Sci, Inst Genom, Key Lab Genom & Precis Med, Beijing 100101, Peoples R China
Recommended Citation
GB/T 7714
Sharshar, Moustafa Mohamed,Samak, Nadia Abdrabo,Hao, Xuemi,et al. Enhanced growth-driven stepwise inducible expression system development in haloalkaliphilic desulfurizing Thioalkalivibrio versutus[J]. BIORESOURCE TECHNOLOGY,2019,288:9.
APA Sharshar, Moustafa Mohamed.,Samak, Nadia Abdrabo.,Hao, Xuemi.,Mu, Tingzhen.,Zhong, Wei.,...&Xing, Jianmin.(2019).Enhanced growth-driven stepwise inducible expression system development in haloalkaliphilic desulfurizing Thioalkalivibrio versutus.BIORESOURCE TECHNOLOGY,288,9.
MLA Sharshar, Moustafa Mohamed,et al."Enhanced growth-driven stepwise inducible expression system development in haloalkaliphilic desulfurizing Thioalkalivibrio versutus".BIORESOURCE TECHNOLOGY 288(2019):9.
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