CAS OpenIR
Effect of in vitro collagen fibrillogenesis on Langmuir-Blodgett (LB) deposition for cellular behavior regulation
Liaw, Bor-Shuang1; Xing, Fangyu2; Wang, Dan1; Gao, Fei3; Lu, Jingsong4; Yu, Jing4; Sun, Xiaodan1; Wang, Xiumei1; Feng, Qingling1; Zhang, Guifeng2; Zhao, Lingyun1
2019-07-01
Source PublicationCOLLOIDS AND SURFACES B-BIOINTERFACES
ISSN0927-7765
Volume179Pages:48-55
AbstractCollagen fibrillogenesis is of special significance for the maintenance of collagen scaffold's mechanical stability and biological performance. Comprehensive information about the mechanism of collagen fibrillogenesis in vitro, as well as the effect of fibrillogenesis on deposited layers of ordered collagen molecules for cellular behavior regulation is thus crucial. In the current study, the pH, phosphate ion as well as reconstitution time impacting on the in vitro fibrillogenesis was systematically investigated, including the zeta potential and turbidity measurement. Furthermore, the fibrillogenesis impacting on the pi-a isotherms of collagen assembly at the air/water interface was then fully evaluated. By applying LB technique, collagen fibril-assembling arrays structure can be successfully transferred to form surface deposition onto the mica and glass substrate. The morphology and collagen content were subsequently assessed by atomic force microscopy (AFM) and hydrolyzing examination respectively. Effect of collagen LB deposition on the adhesion and proliferation of SD rat bone marrow mesenchymal stem cells were estimated by Rhodamine Phalloidin/DIPI staining and CCK8 proliferation assays. The results show that highly oriented and collagen-abundant thin film can further facilitate cell adhesion and proliferation, indicating an innovative direction for tissue engineering.
KeywordCollagen fibrillogenesis Langmuir-Blodgett deposition Self-assembly Cell adhesion Cell proliferation
DOI10.1016/j.colsurfb.2019.03.053
Language英语
WOS KeywordMOLECULAR-INTERACTIONS ; SCHAEFER FILMS ; PACLITAXEL ; ADSORPTION ; MICROSCOPY ; NETWORK ; PH
Funding ProjectIntergovernmental cooperation in science and technology[2016YFE0125300] ; National Natural Science Foundation of China (NSFC)[81671829] ; National Key R&D Program of China[2018YFA0108202] ; National Key R&D Program of China[2018YFC1106402] ; Science and Technology Program of Guangzhou, China[201803010086]
WOS Research AreaBiophysics ; Chemistry ; Materials Science
WOS SubjectBiophysics ; Chemistry, Physical ; Materials Science, Biomaterials
Funding OrganizationIntergovernmental cooperation in science and technology ; National Natural Science Foundation of China (NSFC) ; National Key R&D Program of China ; Science and Technology Program of Guangzhou, China
WOS IDWOS:000471736600006
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/30036
Collection中国科学院过程工程研究所
Corresponding AuthorZhang, Guifeng; Zhao, Lingyun
Affiliation1.Tsinghua Univ, Sch Mat Sci & Engn, State Key Lab New Ceram & Fine Proc, Key Lab Adv Mat,Minist Educ China, Beijing 100084, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
3.Northwest Univ, Coll Chem & Mat Sci, Xian 710069, Shaanxi, Peoples R China
4.Zhejiang Univ Technol, Coll Mat Sci & Engn, Hangzhou 310014, Zhejiang, Peoples R China
Recommended Citation
GB/T 7714
Liaw, Bor-Shuang,Xing, Fangyu,Wang, Dan,et al. Effect of in vitro collagen fibrillogenesis on Langmuir-Blodgett (LB) deposition for cellular behavior regulation[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2019,179:48-55.
APA Liaw, Bor-Shuang.,Xing, Fangyu.,Wang, Dan.,Gao, Fei.,Lu, Jingsong.,...&Zhao, Lingyun.(2019).Effect of in vitro collagen fibrillogenesis on Langmuir-Blodgett (LB) deposition for cellular behavior regulation.COLLOIDS AND SURFACES B-BIOINTERFACES,179,48-55.
MLA Liaw, Bor-Shuang,et al."Effect of in vitro collagen fibrillogenesis on Langmuir-Blodgett (LB) deposition for cellular behavior regulation".COLLOIDS AND SURFACES B-BIOINTERFACES 179(2019):48-55.
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