CAS OpenIR
Establishment and application of a dynamic tumor-vessel microsystem for studying different stages of tumor metastasis and evaluating anti-tumor drugs
Jing, Bolin1,2; Luo, Yong3; Lin, Bingcheng4; Li, Jianjun1; Wang, Zhuo A.1; Du, Yuguang1
2019-06-03
Source PublicationRSC ADVANCES
ISSN2046-2069
Volume9Issue:30Pages:17137-17147
AbstractTumor metastasis is one of the main causes of cancer-related death, and it is difficult to study the whole process of tumor metastasis in vivo due to the complex physiological environment in the body. Therefore, it's crucial to develop simple and physiologically relevant in vitro cancer models to study the metastasis process, especially different phases of tumor metastasis. A novel microfluidic tumor-vessel co-culture system was established to reproduce the different phases of cancer metastasis (proliferation, migration, intravasation and adherence) individually in vitro for the first time. It was observed that blood vessels with fluid flow had big impact on metastasis of liver cancer cells HepG2 and breast ones MDA-MB-231. In particular, it was found that both HepG2 and MDA-MB-231 cells migrated in the direction of "blood flow". Furthermore, MDA-MB-231 cells invaded through paracellular mode disrupting the intercellular endothelial junctions, whereas HepG2 cells engaged in transcellular intravasation through transcellular process. Compared with traditional assays, much more potent inhibition of 5-fluorouracil (5-Fu) on different phases of tumor metastasis was observed on the microsystem. In summary, the microfluidic device yielded abundant information about each phase of tumor metastasis, and would provide a powerful platform for use in drug screening, toxicology studies, and personalized medicine in future.
DOI10.1039/c9ra02069a
Language英语
WOS KeywordENDOTHELIAL BARRIER ; MICROFLUIDIC DEVICE ; INTERSTITIAL FLOW ; CELL INVASION ; CANCER ; MODEL ; MIGRATION ; MICROENVIRONMENT ; PERMEABILITY ; STROMA
Funding ProjectNational Natural Science Foundation of China[31870802]
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
Funding OrganizationNational Natural Science Foundation of China
WOS IDWOS:000471912700022
PublisherROYAL SOC CHEMISTRY
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/30059
Collection中国科学院过程工程研究所
Corresponding AuthorLi, Jianjun; Wang, Zhuo A.; Du, Yuguang
Affiliation1.Chinese Acad Sci, State Key Lab Biochem Engn, Key Lab Biopharmaceut Prod & Formulat Engn, PLA,Inst Proc Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Univ Technol, Sch Pharmaceut Sci & Technol, Dalian, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Jing, Bolin,Luo, Yong,Lin, Bingcheng,et al. Establishment and application of a dynamic tumor-vessel microsystem for studying different stages of tumor metastasis and evaluating anti-tumor drugs[J]. RSC ADVANCES,2019,9(30):17137-17147.
APA Jing, Bolin,Luo, Yong,Lin, Bingcheng,Li, Jianjun,Wang, Zhuo A.,&Du, Yuguang.(2019).Establishment and application of a dynamic tumor-vessel microsystem for studying different stages of tumor metastasis and evaluating anti-tumor drugs.RSC ADVANCES,9(30),17137-17147.
MLA Jing, Bolin,et al."Establishment and application of a dynamic tumor-vessel microsystem for studying different stages of tumor metastasis and evaluating anti-tumor drugs".RSC ADVANCES 9.30(2019):17137-17147.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Jing, Bolin]'s Articles
[Luo, Yong]'s Articles
[Lin, Bingcheng]'s Articles
Baidu academic
Similar articles in Baidu academic
[Jing, Bolin]'s Articles
[Luo, Yong]'s Articles
[Lin, Bingcheng]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Jing, Bolin]'s Articles
[Luo, Yong]'s Articles
[Lin, Bingcheng]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.