CAS OpenIR
Horseradish Peroxidase Immobilized on Multifunctional Hybrid Microspheres for Aflatoxin B1 Removal: Will Enzymatic Reaction be Enhanced by Adsorption?
Zhou, Fangfang1,2; Luo, Jianquan1,2; Qi, Benkun1; Chen, Xiangrong1; Wan, Yinhua1,2
2019-07-10
Source PublicationINDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
ISSN0888-5885
Volume58Issue:27Pages:11710-11719
AbstractAs the most dangerous carcinogens, efficient and green removal of Aflatoxin B1 (AFB1) is imperative. For the first time, horseradish peroxidase (HRP) was immobilized in/on an alginate/chitosan/montmorillonite (SA/CS/MON) hybrid microsphere and applied for the AFB1 removal. Compared to the enzyme immobilization via encapsulation and adsorption followed by cross-linking, HRP immobilized on the microsphere surface via covalent bonding showed the highest specific activity and AFB1 removal efficiency. The negatively charged MON could not only adsorb abundant AFB1 but also attract more CS chains on the microsphere surface and bring more active sites for enzyme immobilization and cross-linking; thus, the SA/CS/MON microspheres had stronger antiswelling ability and higher AFB1 adsorption capacity and enzyme loading. Although the AFB1 enrichment in the hybrid microsphere did not promote its degradation by enzymatic catalysis, the HRP-loaded hybrid microsphere could be reused and the enzymatic degradation of AFB1 increased a little as the adsorption became saturated. The enzymatic reaction was not enhanced by the substrate adsorption in this case because the AFB1 adsorption by the hybrid microspheres would decrease the AFB1 concentration in bulk solution and the degradation products might be adsorbed. The adsorbent (i.e., matrix for enzyme immobilization) has different adsorption ability to the substrate/product, and a catalyst with high activity on the substrate is desirable to maximize the synergistic effect of adsorption and catalysis. The obtained hybrid microsphere prototype is promising as a multifunctional matrix for many purposes since both the adsorbent and the catalyst can be designed.
DOI10.1021/acs.iecr.9b02094
Language英语
WOS KeywordCONTROLLED-RELEASE ; CALCIUM ALGINATE ; B-1 ; DEGRADATION ; DETOXIFICATION ; BEADS ; MEMBRANE ; RICE
Funding ProjectNational Key Research and Development Plan of China[2017YFC1600906] ; Beijing Natural Science Foundation[2192053]
WOS Research AreaEngineering
WOS SubjectEngineering, Chemical
Funding OrganizationNational Key Research and Development Plan of China ; Beijing Natural Science Foundation
WOS IDWOS:000475537000010
PublisherAMER CHEMICAL SOC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/30454
Collection中国科学院过程工程研究所
Corresponding AuthorLuo, Jianquan
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Sch Chem Engn, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Fangfang,Luo, Jianquan,Qi, Benkun,et al. Horseradish Peroxidase Immobilized on Multifunctional Hybrid Microspheres for Aflatoxin B1 Removal: Will Enzymatic Reaction be Enhanced by Adsorption?[J]. INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,2019,58(27):11710-11719.
APA Zhou, Fangfang,Luo, Jianquan,Qi, Benkun,Chen, Xiangrong,&Wan, Yinhua.(2019).Horseradish Peroxidase Immobilized on Multifunctional Hybrid Microspheres for Aflatoxin B1 Removal: Will Enzymatic Reaction be Enhanced by Adsorption?.INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,58(27),11710-11719.
MLA Zhou, Fangfang,et al."Horseradish Peroxidase Immobilized on Multifunctional Hybrid Microspheres for Aflatoxin B1 Removal: Will Enzymatic Reaction be Enhanced by Adsorption?".INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH 58.27(2019):11710-11719.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhou, Fangfang]'s Articles
[Luo, Jianquan]'s Articles
[Qi, Benkun]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhou, Fangfang]'s Articles
[Luo, Jianquan]'s Articles
[Qi, Benkun]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhou, Fangfang]'s Articles
[Luo, Jianquan]'s Articles
[Qi, Benkun]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.