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In Vitro Fabrication and Biocompatibility Assay of a Biomimetic Osteoblastic Niche
Song, Kedong1; Kong, Qian1; Li, Liying1; Wang, Yiwei2; Parungao, Roxanne2; Zheng, Shuangshuang3,4; Nie, Yi3,4; Jiao, Zeren5; Wang, Hongfei6; Liu, Tianqing1
2019-10-01
Source PublicationAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
ISSN0273-2289
Volume189Issue:2Pages:471-484
AbstractA novel HAp-CS/Gel biomimetic osteoblastic niche was fabricated by freeze-drying, and its mechanical strength and biocompatibility were characterized. HAp-CS/Gel scaffolds in various ratios of 100:5, 100:10, and 100:20 (CS/Gel to HAp) were prepared by freeze-drying prior to chemical cross-linking followed by infrared spectrum analysis, EDS, FITR, SEM, fluorescence microscopy, MTT, and ALP experiments. Results from the infrared spectrum analysis showed that HAp doping remained the surface morphology and the architecture of scaffold with interconnected pores in the size range of 135 to 150 mu m. The HAp doping ratio of 100:20 was found to be optimal based on its high porosity of 90%, better water uptake folds of 19.1. In addition, EDS and FITR analyses demonstrated that HAps were uniformly distributed on the surface of a scaffold with aggregates and particles, which has sufficient roughness for cell attachment and proliferation of osteoblasts. Under SEM and fluorescent microscopy, osteoblasts seeded onto the scaffold showed evenly distributed viable cells, which is believed to form a biomimetic niche. In the present study, we further demonstrate that osteoblasts can maintain their function and grow well on the scaffold through MTT and ALP tests. Thus, the scaffold has favorable physical properties and biocompatibility to support the proliferation and differentiation of osteoblasts and further to support the constructs of biomimetic osteoblastic niche.
KeywordOsteoblasts Biomimetic niche Hematopoietic stem cell expansion Dynamic environment Cell function maintain
DOI10.1007/s12010-019-03015-z
Language英语
WOS KeywordHEMATOPOIETIC STEM-CELL ; SCAFFOLDS ; CHITOSAN ; GELATIN ; WOLLASTONITE ; PROTEINS
Funding ProjectNational Natural Science Foundation of China[31670978/31370991/21676041] ; Fok Ying Tung Education Foundation[132027] ; State Key Laboratory of Fine Chemicals[KF1111] ; Natural Science Foundation of Liaoning[20180510028]
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
Funding OrganizationNational Natural Science Foundation of China ; Fok Ying Tung Education Foundation ; State Key Laboratory of Fine Chemicals ; Natural Science Foundation of Liaoning
WOS IDWOS:000487133300011
PublisherSPRINGER
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/31025
Collection中国科学院过程工程研究所
Corresponding AuthorSong, Kedong; Nie, Yi; Wang, Hongfei; Liu, Tianqing
Affiliation1.Dalian Univ Technol, Dalian R&D Ctr Stem Cell & Tissue Engn, State Key Lab Fine Chem, Dalian 116024, Peoples R China
2.Univ Sydney, ANZAC Res Inst, Burns Res Grp, Concord, NSW 2139, Australia
3.Zhengzhou Inst Emerging Technol Ind, Zhengzhou 450000, Henan, Peoples R China
4.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
5.Texas A&M Univ, Artie McFerrin Dept Chem Engn, College Stn, TX 77843 USA
6.Dalian Med Univ, Dept Orthoped, Affiliated Hosp 2, Dalian 116024, Peoples R China
Recommended Citation
GB/T 7714
Song, Kedong,Kong, Qian,Li, Liying,et al. In Vitro Fabrication and Biocompatibility Assay of a Biomimetic Osteoblastic Niche[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2019,189(2):471-484.
APA Song, Kedong.,Kong, Qian.,Li, Liying.,Wang, Yiwei.,Parungao, Roxanne.,...&Liu, Tianqing.(2019).In Vitro Fabrication and Biocompatibility Assay of a Biomimetic Osteoblastic Niche.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,189(2),471-484.
MLA Song, Kedong,et al."In Vitro Fabrication and Biocompatibility Assay of a Biomimetic Osteoblastic Niche".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 189.2(2019):471-484.
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