CAS OpenIR
Fabrication of rigid and macroporous agarose microspheres by pre-cross-linking and surfactant micelles swelling method
Zhao, Xi1; Huang, Lan1,3; Wu, Jie1; Huang, Yong-Dong1; Zhao, Lan1; Wu, Nan1; Zhou, Wei-Qing1; Hao, Dong-Xia1; Ma, Guang-Hui1,2; Su, Zhi-Guo1
2019-10-01
Source PublicationCOLLOIDS AND SURFACES B-BIOINTERFACES
ISSN0927-7765
Volume182Pages:8
AbstractA novel combined method of pre-cross-linking and surfactant micelles swelling was proposed in this study to fabricate highly cross-linked and macroporous agarose (HMA) microspheres. Agarose was chemically modified by allylglycidyl ether (AGE) as heterobifunctional cross-linker via its active glycidyl moieties before gel formation and pre-cross-linking was achieved. By this means, the effective concentration of cross-linker presented in agarose gel increased significantly, and thus cross-linking with a high-efficiency was achieved. Further to enhance the intraparticle mass transfer of agarose microspheres, the surfactant micelles swelling method was utilized to create interconnected macropores. Under the optimal condition, HMA microspheres with homogeneous reticular structure and pore size of hundreds nanometers were successfully prepared. They exhibited a low backpressure with a flow velocity as high as 1987 cm/h, which was much higher than that of commercial Sepharose 4 F F. HMA microspheres were then derivatized with carboxymethyl (CM) groups and applied in ion-exchange chromatography. As expected, CM-HMA column separated model proteins effectively even at a flow velocity three times higher than that of commercial CM-4 F F. Visualization of dynamic protein adsorption by confocal laser scanning microscope (CLSM) revealed that the intraparticle mass transfer of CM-HMA micro spheres was intensified due to its macroporous structure. All of the results indicated the newly developed agarose microspheres were a promising medium for high-speed chromatography.
KeywordAgarose microspheres Pre-cross-linking Surfactant micelles swelling method Macropores High-speed chromatography
DOI10.1016/j.colsurfb.2019.110377
Language英语
WOS KeywordPORE-SIZE DISTRIBUTIONS ; GIGAPOROUS POLYSTYRENE MICROSPHERES ; GEL-BOUND ENZYMES ; SUPERPOROUS AGAROSE ; PERFUSION CHROMATOGRAPHY ; HIGH-CAPACITY ; SEPARATION ; CELLULOSE ; BEADS ; ELECTROPHORESIS
Funding ProjectNational Key Research and Development Program of China[2018YFC0311101] ; National Natural Science Foundation of China (NSFC) project[21821005] ; National Natural Science Foundation of China (NSFC) project[21476241] ; Key Research Program of the Chinese Academy of Sciences[KFZD-SW-218] ; Beijing Municipal Natural Science Foundation of China[2172054]
WOS Research AreaBiophysics ; Chemistry ; Materials Science
WOS SubjectBiophysics ; Chemistry, Physical ; Materials Science, Biomaterials
Funding OrganizationNational Key Research and Development Program of China ; National Natural Science Foundation of China (NSFC) project ; Key Research Program of the Chinese Academy of Sciences ; Beijing Municipal Natural Science Foundation of China
WOS IDWOS:000489190200044
PublisherELSEVIER
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Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/31196
Collection中国科学院过程工程研究所
Corresponding AuthorWu, Jie; Ma, Guang-Hui
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100190, Peoples R China
3.China Univ Min & Technol, Sch Chem & Environm Engn, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Xi,Huang, Lan,Wu, Jie,et al. Fabrication of rigid and macroporous agarose microspheres by pre-cross-linking and surfactant micelles swelling method[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2019,182:8.
APA Zhao, Xi.,Huang, Lan.,Wu, Jie.,Huang, Yong-Dong.,Zhao, Lan.,...&Su, Zhi-Guo.(2019).Fabrication of rigid and macroporous agarose microspheres by pre-cross-linking and surfactant micelles swelling method.COLLOIDS AND SURFACES B-BIOINTERFACES,182,8.
MLA Zhao, Xi,et al."Fabrication of rigid and macroporous agarose microspheres by pre-cross-linking and surfactant micelles swelling method".COLLOIDS AND SURFACES B-BIOINTERFACES 182(2019):8.
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