characterizationofproanthocyaninrelatedleucoanthocyanidinreductaseandanthocyanidinreductasegenesinlyciumruthenicummurr

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	characterizationofproanthocyaninrelatedleucoanthocyanidinreductaseandanthocyanidinreductasegenesinlyciumruthenicummurr
	Shen Xiaofei 1; Zeng Shaohua 2; Wu Min 2; Liu Chunzhao 3; Wang Ying 1
	2014
Source Publication	journalofchinesepharmaceuticalsciences
ISSN	1003-1057
Volume	23 Issue:6 Pages:369
Abstract	Proanthocyanidins (PAs) are a set of important phytochemical compounds in Lycium ruthenicum Murr. However, PA biosynthesis in L. ruthenicum remains unclear. In this study, leucoanthocyanidin reductase (LAR) and anthocyanidin reductases (ANR) involved in PA biosynthesis were retrieved from L. ruthenicum EST database. Polymerase chain reaction (PCR) results confirmed that LrLAR and LrANR encoded a protein composed of 333 and 338 amino acids, respectively. Phylogenetic analysis showed that LrLAR and LrANR were clustered with known LAR and ANR proteins of other plants, respectively. Quantitative reversetranscription PCR (qRT-PCR) results indicated that both genes expressed in a sharp increase manner from unripe stage to color break stage and were downregulated increasingly hereafter, and that both expression levels are extremely low in young leaves, mature leaves, stems, and roots compared with fruits. Phytochemical assay revealed that the content of total PA in fruit was higher than that in young leaves, mature leaves, stems, and roots, and that the total PA level was increased sharply and then relatively stable hereafter during fruit ripening. Our results lay a foundation for uncovering the PA biosynthesis and further engineering manipulation in L. ruthenicum.
Language	英语
Document Type	期刊论文
Identifier	http://ir.ipe.ac.cn/handle/122111/31493
Collection	中国科学院过程工程研究所
Affiliation	1.中国科学院武汉植物园 2.中国科学院华南植物园 3.中国科学院过程工程研究所
Recommended Citation GB/T 7714	Shen Xiaofei,Zeng Shaohua,Wu Min,et al. characterizationofproanthocyaninrelatedleucoanthocyanidinreductaseandanthocyanidinreductasegenesinlyciumruthenicummurr[J]. journalofchinesepharmaceuticalsciences,2014,23(6):369.
APA	Shen Xiaofei,Zeng Shaohua,Wu Min,Liu Chunzhao,&Wang Ying.(2014).characterizationofproanthocyaninrelatedleucoanthocyanidinreductaseandanthocyanidinreductasegenesinlyciumruthenicummurr.journalofchinesepharmaceuticalsciences,23(6),369.
MLA	Shen Xiaofei,et al."characterizationofproanthocyaninrelatedleucoanthocyanidinreductaseandanthocyanidinreductasegenesinlyciumruthenicummurr".journalofchinesepharmaceuticalsciences 23.6(2014):369.

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