CAS OpenIR
characterizationofproanthocyaninrelatedleucoanthocyanidinreductaseandanthocyanidinreductasegenesinlyciumruthenicummurr
Shen Xiaofei1; Zeng Shaohua2; Wu Min2; Liu Chunzhao3; Wang Ying1
2014
Source Publicationjournalofchinesepharmaceuticalsciences
ISSN1003-1057
Volume23Issue:6Pages:369
AbstractProanthocyanidins (PAs) are a set of important phytochemical compounds in Lycium ruthenicum Murr. However, PA biosynthesis in L. ruthenicum remains unclear. In this study, leucoanthocyanidin reductase (LAR) and anthocyanidin reductases (ANR) involved in PA biosynthesis were retrieved from L. ruthenicum EST database. Polymerase chain reaction (PCR) results confirmed that LrLAR and LrANR encoded a protein composed of 333 and 338 amino acids, respectively. Phylogenetic analysis showed that LrLAR and LrANR were clustered with known LAR and ANR proteins of other plants, respectively. Quantitative reversetranscription PCR (qRT-PCR) results indicated that both genes expressed in a sharp increase manner from unripe stage to color break stage and were downregulated increasingly hereafter, and that both expression levels are extremely low in young leaves, mature leaves, stems, and roots compared with fruits. Phytochemical assay revealed that the content of total PA in fruit was higher than that in young leaves, mature leaves, stems, and roots, and that the total PA level was increased sharply and then relatively stable hereafter during fruit ripening. Our results lay a foundation for uncovering the PA biosynthesis and further engineering manipulation in L. ruthenicum.
Language英语
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/31493
Collection中国科学院过程工程研究所
Affiliation1.中国科学院武汉植物园
2.中国科学院华南植物园
3.中国科学院过程工程研究所
Recommended Citation
GB/T 7714
Shen Xiaofei,Zeng Shaohua,Wu Min,et al. characterizationofproanthocyaninrelatedleucoanthocyanidinreductaseandanthocyanidinreductasegenesinlyciumruthenicummurr[J]. journalofchinesepharmaceuticalsciences,2014,23(6):369.
APA Shen Xiaofei,Zeng Shaohua,Wu Min,Liu Chunzhao,&Wang Ying.(2014).characterizationofproanthocyaninrelatedleucoanthocyanidinreductaseandanthocyanidinreductasegenesinlyciumruthenicummurr.journalofchinesepharmaceuticalsciences,23(6),369.
MLA Shen Xiaofei,et al."characterizationofproanthocyaninrelatedleucoanthocyanidinreductaseandanthocyanidinreductasegenesinlyciumruthenicummurr".journalofchinesepharmaceuticalsciences 23.6(2014):369.
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