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Novel thermal-sensitive hydrogel enhances both humoral and cell-mediated immune responses by intranasal vaccine delivery
Wu, Youbin1,2; Wu, Shipo3; Hou, Lihua3; Wei, Wei1; Zhou, Meng1; Su, Zhiguo1; Wu, Jie1; Chen, Wei3; Ma, Guanghui1; Wu, J
2012-08-01
Source PublicationEUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
ISSN0939-6411
Volume81Issue:3Pages:486-497
AbstractA novel thermal sensitive hydrogel was formulated with N-[(2-hydroxy-3-trimethylammonium) propyl] chitosan chloride (HTCC) and alpha, beta-glycerophosphate (alpha, beta-GP). A serial of hydrogels containing different amount of GP and HTCC with diverse quarternize degree (QD, 41%, 59%, 79.5%, and 99%) were prepared and characterized by rheological method. The hydrogel was subsequently evaluated for intranasal vaccine delivery with adenovirus based Zaire Ebola virus glycoprotein antigen (Ad-GPZ). Results showed that moderate quarternized HTCC (60% and 79.5%) hydrogel/antigen formulations induced highest IgG, IgG1, and IgG2a antibody titers in serum, as well as mucosal IgA responses in lung wash, which may attributed to the prolonged antigen residence time due to the thermal-sensitivity of this hydrogel. Furthermore, CD8(+) splenocytes for IFN-gamma positive cell assay and the release profile of Th1/Th2 type cytokines (IFN-gamma, IL-2, IL-10, and IL-4) showed that hydrogel/Ad-GPZ generated an overwhelmingly enhanced Th1 biased cellular immune response. In addition, this hydrogel displayed low toxicity to nasal tissue and epithelial cells even by frequently intranasal dosing of hydrogel. All these results strongly supported this hydrogel as a safe and effective delivery system for nasal immunization. Crown Copyright (C) 2012 Published by Elsevier B.V. All rights reserved.
KeywordQuarternized Chitosan Hydrogel Thermal Sensitive Mucosal Immunization Vaccine Delivery Ebola
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.ejpb.2012.03.021
Indexed BySCI
Language英语
WOS KeywordNASAL DRUG-DELIVERY ; IGG IMMUNOLOGICAL RESPONSES ; CHITOSAN NANOPARTICLES ; INFLUENZA VACCINE ; MUCOSAL SIGA ; EBOLA-VIRUS ; ABSORPTION ENHANCERS ; RHESUS-MONKEYS ; INFECTION ; SYSTEM
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000307040900004
Citation statistics
Cited Times:25[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/3220
Collection生化工程国家重点实验室
Corresponding AuthorWu, J
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China
2.Chinese Acad Sci, Graduated Univ, Beijing, Peoples R China
3.Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogens & Biosecur, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Wu, Youbin,Wu, Shipo,Hou, Lihua,et al. Novel thermal-sensitive hydrogel enhances both humoral and cell-mediated immune responses by intranasal vaccine delivery[J]. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS,2012,81(3):486-497.
APA Wu, Youbin.,Wu, Shipo.,Hou, Lihua.,Wei, Wei.,Zhou, Meng.,...&Wu, J.(2012).Novel thermal-sensitive hydrogel enhances both humoral and cell-mediated immune responses by intranasal vaccine delivery.EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS,81(3),486-497.
MLA Wu, Youbin,et al."Novel thermal-sensitive hydrogel enhances both humoral and cell-mediated immune responses by intranasal vaccine delivery".EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS 81.3(2012):486-497.
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