CAS OpenIR
R-藻红蛋白固相免疫荧光检测中洗涤条件的筛选
顾铭; 吴萍; 欧阳藩; 戚艺华
2001
Source Publication免疫学杂志
ISSN1000-8861
Volume017Issue:003Pages:219
Abstract目的 筛选R-藻红蛋白免疫荧光检测中适宜的洗涤条件。方法 采用二步法改变洗涤液离子强度、pH值,比较弱阳性血清的检出率及阴性假阳性率,由此筛选最佳洗涤条件。结果 洗涤液离子强度的增加有助于降低阴性血清假阳性现象,在本实验所涉及样品范围内,摩尔浓度大于100mmol/L时,假阳性率可降低到0;120mmol/L时,阳性检出率最高值达93.3%。中性、偏碱性的pH值对检出率影响较小。结论 以120mmol/LPBS(pH7.5)为洗涤液有利于提高R-藻红蛋白荧光免疫法的特异性和灵敏度。
Language英语
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/32645
Collection中国科学院过程工程研究所
Affiliation中国科学院过程工程研究所
First Author Affilication中国科学院过程工程研究所
Recommended Citation
GB/T 7714
顾铭,吴萍,欧阳藩,等. R-藻红蛋白固相免疫荧光检测中洗涤条件的筛选[J]. 免疫学杂志,2001,017(003):219.
APA 顾铭,吴萍,欧阳藩,&戚艺华.(2001).R-藻红蛋白固相免疫荧光检测中洗涤条件的筛选.免疫学杂志,017(003),219.
MLA 顾铭,et al."R-藻红蛋白固相免疫荧光检测中洗涤条件的筛选".免疫学杂志 017.003(2001):219.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[顾铭]'s Articles
[吴萍]'s Articles
[欧阳藩]'s Articles
Baidu academic
Similar articles in Baidu academic
[顾铭]'s Articles
[吴萍]'s Articles
[欧阳藩]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[顾铭]'s Articles
[吴萍]'s Articles
[欧阳藩]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.