Liu Zhini1; Yang Liangrong2; Dong Tingting2; Li Wensong1; Sun Xitong2; Zhu Menghao2; Duan Zhengkang1; Liu Qingfen2; Liu Huizhou2
Source Publicationparticuology
AbstractIn this paper, gas-assisted magnetic separation (GAMS), a technique that combines magnetic separation with flotation, was investigated for the potential large-scale separation of proteins. The GAMS process includes adsorption of target proteins and magnetic separation to recover protein-loaded magnetic particles from the dilute biosuspension with the assistance of bubbles. Microsized ethylenediamine-functionalized poly(glycidyl methacrylate) superparamagnetic microspheres (MPMs) and bovine serum albumin (BSA) were used as a model system. The feasibility of GAMS for capturing BSA-loaded MPMs from an appropriate medium was shown. High recovery of BSA-loaded MPMs was obtained by simple adjustment of the initial solution pH without extra detergents and antifoaming agents. The GAMS conditions were consistent with the adsorption conditions, and no proteins were desorbed from the MPMs during this process. Under the optimal conditions, the separation rate and recovery percentage reached 410 mL/min and 98% in 0.61 min, respectively. Conformational changes of BSA during the GAMS process were investigated by fluorescence spectroscopy and circular dichroism spectrometry. (C) 2015 Chinese Society of Particuology and Institute of Process Engineering, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved.
Funding Project[National Key Natural Science Foundation of China] ; [National Natural Science Foundation of China]
Document Type期刊论文
Recommended Citation
GB/T 7714
Liu Zhini,Yang Liangrong,Dong Tingting,et al. gasassistedmagneticseparationforthepurificationofproteinsinbatchsystems[J]. particuology,2016,24(FEB):170.
APA Liu Zhini.,Yang Liangrong.,Dong Tingting.,Li Wensong.,Sun Xitong.,...&Liu Huizhou.(2016).gasassistedmagneticseparationforthepurificationofproteinsinbatchsystems.particuology,24(FEB),170.
MLA Liu Zhini,et al."gasassistedmagneticseparationforthepurificationofproteinsinbatchsystems".particuology 24.FEB(2016):170.
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