过氧化物酶一辅酶nadh催化o2h2o2产生羟基自由基研究及其氯苯处理初探

CAS OpenIR

	过氧化物酶一辅酶nadh催化o2h2o2产生羟基自由基研究及其氯苯处理初探
	李海涛 1; 李玉平 2; 曹宏斌 2; 李鑫钢 1; 薛占强 3
	2010
Source Publication	光谱学与光谱分析
ISSN	1000-0593
Volume	30 Issue:11 Pages:3119
Abstract	The mechanism of radical generation in HRP NADHO(2)/H2O2 systems and state-change of horseradish peroxidase (HRP) was investigated by using ESR and UV measurements and the novel enzyme-coenzymatic systems were performed to de grade chlorobenzene as a non phenolic persistent organic pollutants The UV results showed that compound III was produced from HRP oxidized by hydrogen peroxide with the catalysis of NADH which would generate hydroxyl radical The ESR results demonstrated the production of OH and O-2(-) in enzyme-coenzymatic system in the presence of O-2 or H2O2 with DMPO and POBN as spin-trappers respectively In HRP NADH-H2O2 system compound III was the main state of HRP in the initial 10 min and then converted to HRP with generating hydroxyl radical and after the addition of oxygen the production of hydroxyl radical was promoted rapidly as 4 times as that of the system in absence of oxygen. The addition of SOD(Zn Cu) decreased the production of hydroxyl radical significantly resulting from that SOD eli minated O-2 reduction to O-2(-) by NADH and then inhibited OH formation. The results showed that NADH could improve by about 20% enzyme activity of HRP for phenol removal The removal of chlorobenzene with HRP NADH H2O2 and HRP NADH H2O2 O-2 systems reached 24 6% and 48 2% respectively which was much higher than that with traditional enzymatic system (1 42%) showing a promising prospect in proposal of other non phenol POPs in wastewater
Language	英语
Document Type	期刊论文
Identifier	http://ir.ipe.ac.cn/handle/122111/34840
Collection	中国科学院过程工程研究所
Affiliation	1.天津大学 2.中国科学院过程工程研究所 3.鞍山钢铁集团公司
Recommended Citation GB/T 7714	李海涛,李玉平,曹宏斌,等. 过氧化物酶一辅酶nadh催化o2h2o2产生羟基自由基研究及其氯苯处理初探[J]. 光谱学与光谱分析,2010,30(11):3119.
APA	李海涛,李玉平,曹宏斌,李鑫钢,&薛占强.(2010).过氧化物酶一辅酶nadh催化o2h2o2产生羟基自由基研究及其氯苯处理初探.光谱学与光谱分析,30(11),3119.
MLA	李海涛,et al."过氧化物酶一辅酶nadh催化o2h2o2产生羟基自由基研究及其氯苯处理初探".光谱学与光谱分析 30.11(2010):3119.

Files in This Item:
There are no files associated with this item.

If you have any objections to this item, please fill out the form below and the administrator will contact you as soon as possible.
Content:
Email：	*
Institution:
Verification Code:	Refresh

Any comments and suggestions are welcomed.
Title:	*
Content:
Email：	*
Verification Code:	Refresh

中国科学院过程工程研究所机构知识库