李海涛1; 李玉平2; 曹宏斌2; 李鑫钢1; 薛占强3
Source Publication光谱学与光谱分析
AbstractThe mechanism of radical generation in HRP NADHO(2)/H2O2 systems and state-change of horseradish peroxidase (HRP) was investigated by using ESR and UV measurements and the novel enzyme-coenzymatic systems were performed to de grade chlorobenzene as a non phenolic persistent organic pollutants The UV results showed that compound III was produced from HRP oxidized by hydrogen peroxide with the catalysis of NADH which would generate hydroxyl radical The ESR results demonstrated the production of OH and O-2(-) in enzyme-coenzymatic system in the presence of O-2 or H2O2 with DMPO and POBN as spin-trappers respectively In HRP NADH-H2O2 system compound III was the main state of HRP in the initial 10 min and then converted to HRP with generating hydroxyl radical and after the addition of oxygen the production of hydroxyl radical was promoted rapidly as 4 times as that of the system in absence of oxygen. The addition of SOD(Zn Cu) decreased the production of hydroxyl radical significantly resulting from that SOD eli minated O-2 reduction to O-2(-) by NADH and then inhibited OH formation. The results showed that NADH could improve by about 20% enzyme activity of HRP for phenol removal The removal of chlorobenzene with HRP NADH H2O2 and HRP NADH H2O2 O-2 systems reached 24 6% and 48 2% respectively which was much higher than that with traditional enzymatic system (1 42%) showing a promising prospect in proposal of other non phenol POPs in wastewater
Document Type期刊论文
Recommended Citation
GB/T 7714
李海涛,李玉平,曹宏斌,等. 过氧化物酶一辅酶nadh催化o2h2o2产生羟基自由基研究及其氯苯处理初探[J]. 光谱学与光谱分析,2010,30(11):3119.
APA 李海涛,李玉平,曹宏斌,李鑫钢,&薛占强.(2010).过氧化物酶一辅酶nadh催化o2h2o2产生羟基自由基研究及其氯苯处理初探.光谱学与光谱分析,30(11),3119.
MLA 李海涛,et al."过氧化物酶一辅酶nadh催化o2h2o2产生羟基自由基研究及其氯苯处理初探".光谱学与光谱分析 30.11(2010):3119.
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