CAS OpenIR
Simultaneous cell disruption and aqueous two phase extraction for isolation of intracellular recombinant proteins
Feng XL(冯小黎)1; Wang GH(王光虎)1; Su ZG(苏志国)2
1999
Source PublicationChinese Journal of Chemical Engineering
ISSN1004-9541
Volume7Issue:2Pages:139
AbstractA new technique was developed for the integrated processing of cell disruption and aqueous two-phase extraction in a high-speed bead mill to separate intracellular proteins from microbial cells. The process was named as simultaneous cell disruption and aqueous two-phase extraction (SDATE). Advantages, such as high cell disruption efficiency, biochemical activities preservation of proteins, cell debris elimination, and preliminary purification of the target protein were being claimed. When this technique was employed for isolating recombinant Tumor Necrosis Factor (TNF) from E. coli, overall protein concentration and TNF activity were found to have been increased. More than 95% of TNF was partitioned into the top phase and all cell debris were in the bottom phase. The partition coefficient was greater than 3 and the TNF purification factor was greater than 6. It is shown that less separation steps were being utilized in the new technique, meaning a reduction in separation time and less process extractors required.
Language英语
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/37272
Collection中国科学院过程工程研究所
Affiliation1.大连理工大学
2.中国科学院过程工程研究所
Recommended Citation
GB/T 7714
Feng XL,Wang GH,Su ZG. Simultaneous cell disruption and aqueous two phase extraction for isolation of intracellular recombinant proteins[J]. Chinese Journal of Chemical Engineering,1999,7(2):139.
APA 冯小黎,王光虎,&苏志国.(1999).Simultaneous cell disruption and aqueous two phase extraction for isolation of intracellular recombinant proteins.Chinese Journal of Chemical Engineering,7(2),139.
MLA 冯小黎,et al."Simultaneous cell disruption and aqueous two phase extraction for isolation of intracellular recombinant proteins".Chinese Journal of Chemical Engineering 7.2(1999):139.
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