Knowledge Management System Of Institute of process engineering,CAS
Simultaneous cell disruption and aqueous two phase extraction for isolation of intracellular recombinant proteins | |
Feng XL(冯小黎)1; Wang GH(王光虎)1; Su ZG(苏志国)2 | |
1999 | |
Source Publication | Chinese Journal of Chemical Engineering
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ISSN | 1004-9541 |
Volume | 7Issue:2Pages:139 |
Abstract | A new technique was developed for the integrated processing of cell disruption and aqueous two-phase extraction in a high-speed bead mill to separate intracellular proteins from microbial cells. The process was named as simultaneous cell disruption and aqueous two-phase extraction (SDATE). Advantages, such as high cell disruption efficiency, biochemical activities preservation of proteins, cell debris elimination, and preliminary purification of the target protein were being claimed. When this technique was employed for isolating recombinant Tumor Necrosis Factor (TNF) from E. coli, overall protein concentration and TNF activity were found to have been increased. More than 95% of TNF was partitioned into the top phase and all cell debris were in the bottom phase. The partition coefficient was greater than 3 and the TNF purification factor was greater than 6. It is shown that less separation steps were being utilized in the new technique, meaning a reduction in separation time and less process extractors required. |
Language | 英语 |
Document Type | 期刊论文 |
Identifier | http://ir.ipe.ac.cn/handle/122111/37272 |
Collection | 中国科学院过程工程研究所 |
Affiliation | 1.大连理工大学 2.中国科学院过程工程研究所 |
Recommended Citation GB/T 7714 | Feng XL,Wang GH,Su ZG. Simultaneous cell disruption and aqueous two phase extraction for isolation of intracellular recombinant proteins[J]. Chinese Journal of Chemical Engineering,1999,7(2):139. |
APA | 冯小黎,王光虎,&苏志国.(1999).Simultaneous cell disruption and aqueous two phase extraction for isolation of intracellular recombinant proteins.Chinese Journal of Chemical Engineering,7(2),139. |
MLA | 冯小黎,et al."Simultaneous cell disruption and aqueous two phase extraction for isolation of intracellular recombinant proteins".Chinese Journal of Chemical Engineering 7.2(1999):139. |
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