Simultaneous cell disruption and aqueous two phase extraction for isolation of intracellular recombinant proteins

CAS OpenIR

	Simultaneous cell disruption and aqueous two phase extraction for isolation of intracellular recombinant proteins
	Feng XL(冯小黎)1; Wang GH(王光虎)1; Su ZG(苏志国)2
	1999
Source Publication	Chinese Journal of Chemical Engineering
ISSN	1004-9541
Volume	7 Issue:2 Pages:139
Abstract	A new technique was developed for the integrated processing of cell disruption and aqueous two-phase extraction in a high-speed bead mill to separate intracellular proteins from microbial cells. The process was named as simultaneous cell disruption and aqueous two-phase extraction (SDATE). Advantages, such as high cell disruption efficiency, biochemical activities preservation of proteins, cell debris elimination, and preliminary purification of the target protein were being claimed. When this technique was employed for isolating recombinant Tumor Necrosis Factor (TNF) from E. coli, overall protein concentration and TNF activity were found to have been increased. More than 95% of TNF was partitioned into the top phase and all cell debris were in the bottom phase. The partition coefficient was greater than 3 and the TNF purification factor was greater than 6. It is shown that less separation steps were being utilized in the new technique, meaning a reduction in separation time and less process extractors required.
Language	英语
Document Type	期刊论文
Identifier	http://ir.ipe.ac.cn/handle/122111/37272
Collection	中国科学院过程工程研究所
Affiliation	1.大连理工大学 2.中国科学院过程工程研究所
Recommended Citation GB/T 7714	Feng XL,Wang GH,Su ZG. Simultaneous cell disruption and aqueous two phase extraction for isolation of intracellular recombinant proteins[J]. Chinese Journal of Chemical Engineering,1999,7(2):139.
APA	冯小黎,王光虎,&苏志国.(1999).Simultaneous cell disruption and aqueous two phase extraction for isolation of intracellular recombinant proteins.Chinese Journal of Chemical Engineering,7(2),139.
MLA	冯小黎,et al."Simultaneous cell disruption and aqueous two phase extraction for isolation of intracellular recombinant proteins".Chinese Journal of Chemical Engineering 7.2(1999):139.

Files in This Item:
There are no files associated with this item.

If you have any objections to this item, please fill out the form below and the administrator will contact you as soon as possible.
Content:
Email：	*
Institution:
Verification Code:	Refresh

Any comments and suggestions are welcomed.
Title:	*
Content:
Email：	*
Verification Code:	Refresh

中国科学院过程工程研究所机构知识库