CAS OpenIR
大肠杆菌ecolihb101pbr322高密度培养过程质粒的稳定性
王骥程1; 喻国策2; 王树青1; 焦瑞身3
2001
Source Publication过程工程学报
ISSN1009-606X
Volume001Issue:002Pages:185
Abstract通过正交实验考察了大肠杆菌E.coli HB101(pBR322)高密度培养过程中,不同培养条件对质粒的稳定性和β-内酰胺酶活力的影响。结果表明,当温度在33-39℃、pH为6.4-7.2、DO为40%0-80%、补料速率在5.4-10.8g/h范围内变化,以及细胞密度达到27.3g/L、比生长速率达到0.73h^-1时,质粒没有发生丢失现象,但在培养过程中β-内酰胺酶的活力降低。
Language英语
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/38079
Collection中国科学院过程工程研究所
Affiliation1.浙江大学
2.中国科学院过程工程研究所
3.中国科学院上海生命科学研究院
Recommended Citation
GB/T 7714
王骥程,喻国策,王树青,等. 大肠杆菌ecolihb101pbr322高密度培养过程质粒的稳定性[J]. 过程工程学报,2001,001(002):185.
APA 王骥程,喻国策,王树青,&焦瑞身.(2001).大肠杆菌ecolihb101pbr322高密度培养过程质粒的稳定性.过程工程学报,001(002),185.
MLA 王骥程,et al."大肠杆菌ecolihb101pbr322高密度培养过程质粒的稳定性".过程工程学报 001.002(2001):185.
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