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Sensitive and selective fluorometric determination of DNA by using layered hexagonal nanosheets of a covalent organic framework prepared from p-phenylenediamine and benzene-1,3,5-tricarboxaldehyde
Mandal, Tapas Kumar1; Parvin, Nargish2; Mishra, Kanchan1; Mohandoss, Sonaimuthu1; Lee, Yong Rok1
2019-12-01
Source PublicationMICROCHIMICA ACTA
ISSN0026-3672
Volume186Issue:12Pages:8
AbstractA modified method is described for the preparation of amino-functionalized covalent organic framework nanosheets (COF-NSs). These consist of hexagonal layered sheets and were prepared from commercially available starting materials (p-phenylenediamine and benzene-1,3,5-tricarboxaldehyde). The interlayer stacking interactions between the ultra-thin COF-NSs became weak because the pi stacking is destroyed by sonication. This result in the exfoliation of COF-NSs. As an application, the COF-NSs used for sensitive and selective fluorometric determination of DNA. To reach this goal, H1 and H2 hairpin-like DNA probes were chosen; H1 used Texas Red-labeled dye as a fluorescent probe. The addition of the COF-NSs, the hairpin probes was adsorbed onto the porous surface of the COFNSs. The pi stacking and hydrogen-bond interactions between COFNSs and nucleic acid quench the fluorescence of the Texas red-labeled probe. The target DNA enables the recovery of the quenched fluorescence of the Texas red-labelled probe by triggering an inter-chain hybridization within hairpin probes. This results in a weaker interaction of double-stranded DNA (dsDNA) with the COFNSs. Consequently, the dsDNA detaches from the COFNSs, thereby recovering the dye's fluorescence (excitation/emission maxima at 590/612 nm) with increasing target DNA concentration. The findings were applied to design a method for the determination of DNA that has a 2 pM detection limit. This is significantly lower than the limit of detection reported previously for 2D nanomaterial-based fluorometric DNA assays.
KeywordPorous nanomaterials Bond Fluorescence Signal Target Biomolecules Biomarker Diseases Sensor Diagnostics
DOI10.1007/s00604-019-3944-1
Language英语
WOS KeywordGRAPHENE OXIDE
Funding ProjectNational Research Foundation of Korea (NRF) - Korea government (MSIT)[2018R1A2B2004432]
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
Funding OrganizationNational Research Foundation of Korea (NRF) - Korea government (MSIT)
WOS IDWOS:000499967800017
PublisherSPRINGER WIEN
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/38340
Collection中国科学院过程工程研究所
Corresponding AuthorMandal, Tapas Kumar
Affiliation1.Yeungnam Univ, Sch Chem Engn, Gyongsan 38541, South Korea
2.Chinese Acad Sci, Inst Proc Engn, CAS Ctr Excellence Nanosci, State Key Lab Biochem Engn, 1 Beiertiao, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Mandal, Tapas Kumar,Parvin, Nargish,Mishra, Kanchan,et al. Sensitive and selective fluorometric determination of DNA by using layered hexagonal nanosheets of a covalent organic framework prepared from p-phenylenediamine and benzene-1,3,5-tricarboxaldehyde[J]. MICROCHIMICA ACTA,2019,186(12):8.
APA Mandal, Tapas Kumar,Parvin, Nargish,Mishra, Kanchan,Mohandoss, Sonaimuthu,&Lee, Yong Rok.(2019).Sensitive and selective fluorometric determination of DNA by using layered hexagonal nanosheets of a covalent organic framework prepared from p-phenylenediamine and benzene-1,3,5-tricarboxaldehyde.MICROCHIMICA ACTA,186(12),8.
MLA Mandal, Tapas Kumar,et al."Sensitive and selective fluorometric determination of DNA by using layered hexagonal nanosheets of a covalent organic framework prepared from p-phenylenediamine and benzene-1,3,5-tricarboxaldehyde".MICROCHIMICA ACTA 186.12(2019):8.
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