3D Bio-Printing of CS/Gel/HA/Gr Hybrid Osteochondral Scaffolds
Hu, Xueyan1; Man, Yuan1; Li, Wenfang1; Li, Liying1; Xu, Jie1; Parungao, Roxanne2; Wang, Yiwei2; Zheng, Shuangshuang3; Nie, Yi3,4; Liu, Tianqing1; Song, Kedong1
Source PublicationPOLYMERS

Cartilage is an important tissue contributing to the structure and function of support and protection in the human body. There are many challenges for tissue cartilage repair. However, 3D bio-printing of osteochondral scaffolds provides a promising solution. This study involved preparing bio-inks with different proportions of chitosan (Cs), Gelatin (Gel), and Hyaluronic acid (HA). The rheological properties of each bio-ink was used to identify the optimal bio-ink for printing. To improve the mechanical properties of the bio-scaffold, Graphene (GR) with a mass ratio of 0.024, 0.06, and 0.1% was doped in the bio-ink. Bio-scaffolds were prepared using 3D printing technology. The mechanical strength, water absorption rate, porosity, and degradation rate of the bio-scaffolds were compared to select the most suitable scaffold to support the proliferation and differentiation of cells. P3 Bone mesenchymal stem cells (BMSCs) were inoculated onto the bio-scaffolds to study the biocompatibility of the scaffolds. The results of SEM showed that the Cs/Gel/HA scaffolds with a GR content of 0, 0.024, 0.06, and 0.1% had a good three-dimensional porous structure and interpenetrating pores, and a porosity of more than 80%. GR was evenly distributed on the scaffold as observed by energy spectrum analyzer and polarizing microscope. With increasing GR content, the mechanical strength of the scaffold was enhanced, and pore walls became thicker and smoother. BMSCs were inoculated on the different scaffolds. The cells distributed and extended well on Cs/Gel/HA/GR scaffolds. Compared to traditional methods in tissue-engineering, this technique displays important advantages in simulating natural cartilage with the ability to finely control the mechanical and chemical properties of the scaffold to support cell distribution and proliferation for tissue repair.

Keyword3d Printing Bio-ink Graphene Chitosan Gelatin Hyaluronic Acid Cartilage Repair
WOS KeywordArticular-cartilage Defects ; Chondrogenic Differentiation ; Tissue Constructs ; Drug-delivery ; Hydrogel ; Chitosan ; Nanomaterials ; Repair ; Therapy ; Matrix
Funding ProjectNational Natural Science Foundation of China[31670978/31370991/21676041] ; Fok Ying Tung Education Foundation[132027] ; State Key Laboratory of Fine Chemicals[KF1111] ; Natural Science Foundation of Liaoning[20180510028]
WOS Research AreaPolymer Science
WOS SubjectPolymer Science
Funding OrganizationNational Natural Science Foundation of China ; Fok Ying Tung Education Foundation ; State Key Laboratory of Fine Chemicals ; Natural Science Foundation of Liaoning
WOS IDWOS:000495382700070
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Document Type期刊论文
Corresponding AuthorNie, Yi; Liu, Tianqing; Song, Kedong
Affiliation1.Dalian Univ Technol, Dalian R&D Ctr Stem Cell & Tissue Engn, State Key Lab Fine Chem, Dalian 116024, Peoples R China
2.Univ Sydney, ANZAC Res Inst, Burns Res Grp, Concord, NSW 2139, Australia
3.Zhengzhou Inst Emerging Ind Technol, Zhengzhou 450000, Henan, Peoples R China
4.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Hu, Xueyan,Man, Yuan,Li, Wenfang,et al. 3D Bio-Printing of CS/Gel/HA/Gr Hybrid Osteochondral Scaffolds[J]. POLYMERS,2019,11(10):16.
APA Hu, Xueyan.,Man, Yuan.,Li, Wenfang.,Li, Liying.,Xu, Jie.,...&Song, Kedong.(2019).3D Bio-Printing of CS/Gel/HA/Gr Hybrid Osteochondral Scaffolds.POLYMERS,11(10),16.
MLA Hu, Xueyan,et al."3D Bio-Printing of CS/Gel/HA/Gr Hybrid Osteochondral Scaffolds".POLYMERS 11.10(2019):16.
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