Knowledge Management System Of Institute of process engineering,CAS
| Fabrication of poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) macroporous microspheres through activators regenerated by electron transfer atom transfer radical polymerization for rapid separation of proteins | |
| Dai, Ning2; Wang, Shaoyun4; Li, Heng1; Zhao, Lan3; Jin, Haibo1; An, Ning1; Gong, Pisheng1; Tan, Qiqi1; Tang, Xu1; Wang, Fei1; Zhang, Rongyue1 | |
| 2019-10-01 | |
| Source Publication | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| ISSN | 1570-0232 |
| Volume | 1128Pages:10 |
| Abstract | Activators regenerated by electron transfer atom transfer radical polymerization (AGET ATRP) were firstly used in suspension polymerization to prepare macroporous microspheres based on a copolymer of glycidyl methacrylate and ethylene glycol dimethacrylate. Compared to conventional radical polymerization (CRP), the microspheres by AGET ATRP showed more homogeneous structure, larger pores, and higher protein binding capacity. The body of microspheres are formed by the large clusters resulted from the aggregated little particles. The size of the particles in microspheres by AGET ATRP was 10-300 nm which was smaller than that (400-800 nm) of the microspheres by CRP. AGET ATRP gave larger pore size (275 +/- 5 nm) and surface area (59.3 +/- 1 m(2)/g) than CRP (234 +/- 5 nm, 37.5 +/- 1 m(2)/g). The microspheres were modified with polyethylene imine for anion resins that were evaluated in term of its protein binding capacity. The results indicated that the static (69 +/- 0.5 mg/mL) and dynamic binding capacity (61 +/- 0.5 mg/mL) of proteins on modified microspheres by AGET ATRP were higher than that (34 +/- 0.5 mg/mL and 19 +/- 0.5 mg/mL) by CRP. Meanwhile, the proteins binding capacity on the microspheres by AGET ATRP decreased only less than 10% when the flow rate increased 10 times. These macroporous media show a large potential in rapid separation of proteins. |
| Keyword | Macroporous microspheres Suspension polymerization Atom transfer radical polymerization Separation of proteins |
| DOI | 10.1016/j.jchromb.2019.121794 |
| Language | 英语 |
| WOS Keyword | ION-EXCHANGER ; HIGH-CAPACITY ; PORE-SIZE ; ADSORPTION ; PARTICLES ; POROGEN ; RESINS ; MEDIA ; BEADS |
| Funding Project | Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges under Beijing Municipality[IDHT20180508] ; Beijing Natural Science Foundation[2162013] ; Beijing Natural Science Foundation[2172054] ; Construction of Scientific Research Platform[2018XK002] |
| WOS Research Area | Biochemistry & Molecular Biology ; Chemistry |
| WOS Subject | Biochemical Research Methods ; Chemistry, Analytical |
| Funding Organization | Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges under Beijing Municipality ; Beijing Natural Science Foundation ; Construction of Scientific Research Platform |
| WOS ID | WOS:000493218700018 |
| Publisher | ELSEVIER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/38961 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Zhang, Rongyue |
| Affiliation | 1.Beijing Inst Petrochem Technol, Coll Chem Engn, Beijing Key Lab Fuels Cleaning & Adv Catalyt Emis, Beijing 102617, Peoples R China 2.Capital Med Univ, Beijing Friendship Hosp, Beijing 100050, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 4.Senhui Microsphere Tech Suzhou Co Ltd, Suzhou 215123, Peoples R China |
| Recommended Citation GB/T 7714 | Dai, Ning,Wang, Shaoyun,Li, Heng,et al. Fabrication of poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) macroporous microspheres through activators regenerated by electron transfer atom transfer radical polymerization for rapid separation of proteins[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2019,1128:10. |
| APA | Dai, Ning.,Wang, Shaoyun.,Li, Heng.,Zhao, Lan.,Jin, Haibo.,...&Zhang, Rongyue.(2019).Fabrication of poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) macroporous microspheres through activators regenerated by electron transfer atom transfer radical polymerization for rapid separation of proteins.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1128,10. |
| MLA | Dai, Ning,et al."Fabrication of poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) macroporous microspheres through activators regenerated by electron transfer atom transfer radical polymerization for rapid separation of proteins".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1128(2019):10. |
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