CAS OpenIR
Fabrication of poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) macroporous microspheres through activators regenerated by electron transfer atom transfer radical polymerization for rapid separation of proteins
Dai, Ning2; Wang, Shaoyun4; Li, Heng1; Zhao, Lan3; Jin, Haibo1; An, Ning1; Gong, Pisheng1; Tan, Qiqi1; Tang, Xu1; Wang, Fei1; Zhang, Rongyue1
2019-10-01
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN1570-0232
Volume1128Pages:10
AbstractActivators regenerated by electron transfer atom transfer radical polymerization (AGET ATRP) were firstly used in suspension polymerization to prepare macroporous microspheres based on a copolymer of glycidyl methacrylate and ethylene glycol dimethacrylate. Compared to conventional radical polymerization (CRP), the microspheres by AGET ATRP showed more homogeneous structure, larger pores, and higher protein binding capacity. The body of microspheres are formed by the large clusters resulted from the aggregated little particles. The size of the particles in microspheres by AGET ATRP was 10-300 nm which was smaller than that (400-800 nm) of the microspheres by CRP. AGET ATRP gave larger pore size (275 +/- 5 nm) and surface area (59.3 +/- 1 m(2)/g) than CRP (234 +/- 5 nm, 37.5 +/- 1 m(2)/g). The microspheres were modified with polyethylene imine for anion resins that were evaluated in term of its protein binding capacity. The results indicated that the static (69 +/- 0.5 mg/mL) and dynamic binding capacity (61 +/- 0.5 mg/mL) of proteins on modified microspheres by AGET ATRP were higher than that (34 +/- 0.5 mg/mL and 19 +/- 0.5 mg/mL) by CRP. Meanwhile, the proteins binding capacity on the microspheres by AGET ATRP decreased only less than 10% when the flow rate increased 10 times. These macroporous media show a large potential in rapid separation of proteins.
KeywordMacroporous microspheres Suspension polymerization Atom transfer radical polymerization Separation of proteins
DOI10.1016/j.jchromb.2019.121794
Language英语
WOS KeywordION-EXCHANGER ; HIGH-CAPACITY ; PORE-SIZE ; ADSORPTION ; PARTICLES ; POROGEN ; RESINS ; MEDIA ; BEADS
Funding ProjectProject of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges under Beijing Municipality[IDHT20180508] ; Beijing Natural Science Foundation[2162013] ; Beijing Natural Science Foundation[2172054] ; Construction of Scientific Research Platform[2018XK002]
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
Funding OrganizationProject of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges under Beijing Municipality ; Beijing Natural Science Foundation ; Construction of Scientific Research Platform
WOS IDWOS:000493218700018
PublisherELSEVIER
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/38961
Collection中国科学院过程工程研究所
Corresponding AuthorZhang, Rongyue
Affiliation1.Beijing Inst Petrochem Technol, Coll Chem Engn, Beijing Key Lab Fuels Cleaning & Adv Catalyt Emis, Beijing 102617, Peoples R China
2.Capital Med Univ, Beijing Friendship Hosp, Beijing 100050, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
4.Senhui Microsphere Tech Suzhou Co Ltd, Suzhou 215123, Peoples R China
Recommended Citation
GB/T 7714
Dai, Ning,Wang, Shaoyun,Li, Heng,et al. Fabrication of poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) macroporous microspheres through activators regenerated by electron transfer atom transfer radical polymerization for rapid separation of proteins[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2019,1128:10.
APA Dai, Ning.,Wang, Shaoyun.,Li, Heng.,Zhao, Lan.,Jin, Haibo.,...&Zhang, Rongyue.(2019).Fabrication of poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) macroporous microspheres through activators regenerated by electron transfer atom transfer radical polymerization for rapid separation of proteins.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1128,10.
MLA Dai, Ning,et al."Fabrication of poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) macroporous microspheres through activators regenerated by electron transfer atom transfer radical polymerization for rapid separation of proteins".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1128(2019):10.
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