Knowledge Management System Of Institute of process engineering,CAS
| Extraction, Characterization, Antitumor and Immunological Activities of Hemicellulose Polysaccharide from Astragalus radix Herb Residue | |
| Li, Ke1,2; Li, Shuying1,2,3; Wang, Di1,2,3; Li, Xiaoxia4; Wu, Xingkang1,2; Liu, Xiaojie1,2; Du, Guanhua5; Li, Xianrong6,7; Qin, Xuemei1,2; Du, Yuguang8 | |
| 2019-10-01 | |
| Source Publication | MOLECULES
 |
| Volume | 24Issue:20Pages:21 |
| Abstract | Astragalus radix (radix) have been frequently used for clinical application in China, and the herb residues of radix turn out to be a waste of resources. To escape from this, the medicine value of radix herb residues is mined in this article. We isolated hemicellulose polysaccharide AX-I-3b from radix herb residues by fractional extraction. Monosaccharide-composition analysis revealed that AX-I-3b consisted of arabinose, xylose, and glucose with a molar ratio of 10.4:79.3:1.1. Methylation, NMR and FT-IR analyses showed that AX-I-3b monosaccharide residue was linked as follows: -> 2,3,4)-beta -d-Xylp-(1 ->, -> 4)-beta -d-Arap-(1 ->, -> 4)-beta -d-Glcp-(1 ->. Then, we found that AX-I-3b exhibited antitumor activity against lung cancer in vitro and vivo through MTT assay and xenograft tumor model. Mechanistically, AX-I-3b induced apoptosis in lung cancer cells and xenograft tumors, which is evidenced by the up-regulation of p53, Bax and cleaved caspase-3, and the down-regulation of Bcl-2. Moreover, AX-I-3b synergistically improved the therapeutic ability of cisplatin in xenograft tumors model. Furthermore, AX-I-3b treatment effectively improved the immune organ index, the percentage of spleen lymphocyte subsets and serum cytokine levels in lung cancer mice, supporting that AX-I-3b showed immunomodulatory activity. In conclusion, our results identified AX-I-3b as an antitumor and immunomodulatory agent, providing a new insight into the reutilization of radix herb residue. |
| Keyword | Astragalus radix herb residue hemicellulose polysaccharide characterization antitumor immunomodulatory |
| DOI | 10.3390/molecules24203644 |
| Language | 英语 |
| WOS Keyword | STRUCTURAL-CHARACTERIZATION ; BCL-2 FAMILY ; IMMUNOMODULATORY ACTIVITY ; ANTIOXIDANT ACTIVITY ; IN-VITRO ; T-CELLS ; P53 ; APOPTOSIS ; MEMBERS ; ALKALI |
| Funding Project | National Natural Science Foundation of China[81872962] ; China Postdoctoral Science Foundation[2019M650851] ; Science and Technology Research Project of Shanxi Province[2014ZD0401] ; Key Projects of Key Research and Development Plan in Shanxi[201603D311101] ; Shanxi Province Technology Innovation Project of Excellent Talent[201605D211030] ; Shanxi Province Technology Innovation Project of Excellent Talent[201705D211020] |
| WOS Research Area | Biochemistry & Molecular Biology ; Chemistry |
| WOS Subject | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| Funding Organization | National Natural Science Foundation of China ; China Postdoctoral Science Foundation ; Science and Technology Research Project of Shanxi Province ; Key Projects of Key Research and Development Plan in Shanxi ; Shanxi Province Technology Innovation Project of Excellent Talent |
| WOS ID | WOS:000496249500013 |
| Publisher | MDPI |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/39117 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Qin, Xuemei; Du, Yuguang |
| Affiliation | 1.Shanxi Univ, Modern Res Ctr Tradit Chinese Med, Taiyuan 030006, Shanxi, Peoples R China 2.Shanxi Univ, Engn Minist, Key Lab Chem Biol & Mol, Taiyuan 030006, Shanxi, Peoples R China 3.Shanxi Univ, Coll Chem & Chem Engn, Taiyuan 030006, Shanxi, Peoples R China 4.Shanxi Fruit Ind Work Stn, Taiyuan 030001, Shanxi, Peoples R China 5.Chinese Acad Med Sci, Inst Mat Med, Beijing 100050, Peoples R China 6.Shanxi Jianshuo Food & Drug Res Inst Co Ltd, Taiyuan 030000, Shanxi, Peoples R China 7.Shanxi Acad Tradit Chinese Med, Taiyuan 030000, Shanxi, Peoples R China 8.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China |
| Recommended Citation GB/T 7714 | Li, Ke,Li, Shuying,Wang, Di,et al. Extraction, Characterization, Antitumor and Immunological Activities of Hemicellulose Polysaccharide from Astragalus radix Herb Residue[J]. MOLECULES,2019,24(20):21. |
| APA | Li, Ke.,Li, Shuying.,Wang, Di.,Li, Xiaoxia.,Wu, Xingkang.,...&Du, Yuguang.(2019).Extraction, Characterization, Antitumor and Immunological Activities of Hemicellulose Polysaccharide from Astragalus radix Herb Residue.MOLECULES,24(20),21. |
| MLA | Li, Ke,et al."Extraction, Characterization, Antitumor and Immunological Activities of Hemicellulose Polysaccharide from Astragalus radix Herb Residue".MOLECULES 24.20(2019):21. |
| Files in This Item: | There are no files associated with this item. | |||||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment