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Fabrication of macroporous microspheres with core-shell structure for negative chromatography purification of virus
An, Ning1; Gong, Pisheng1; Hou, Henglei1; Chi, Weiya1; Jin, Haibo1; Zhao, Lan2; Tan, Qiqi1; Tang, Xu1; Wang, Fei1; Jin, Hongchao1; Zhang, Rongyue1
2020-01-11
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1610Pages:4
AbstractThe macroporous microspheres with core-shell structure, based on a copolymer of 4-Vinylbenzyl chloride, glycidyl methacrylate, and ethylene glycol dimethacrylate, were fabricated through atom transfer radical polymerization suspension polymerization. The microspheres showed 100-200 nm pores in shell and 500-900 nm pores in core. The shell was hydrophilic modified through grafting of poly(N-hydroxyethyl acrylamide) onto the shell surface for reducing adsorption of proteins. The core was coupled with a ligand of poly(ethylene imine) that could bind the proteins. Feedstock of avian influenza virus could be purified on these modified microspheres through negative chromatography. Avian influenza virus cannot enter the core and was recovered from the flow-through, while other proteins with negative charges were able to penetrate into the core and bind to the poly(ethylene imine) ligands. The dynamic binding capacity of proteins was higher on this medium (61 mg/mL) than the commercially available resin (12 mg/mL, Capto Core 700). (C) 2019 Elsevier B.V. All rights reserved.
KeywordNegative chromatography Macroporous microsphere with core-shell structure Purification of virus Avian influenza virus
DOI10.1016/j.chroma.2019.460578
Language英语
WOS KeywordPROTEIN
Funding ProjectProject of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges under Beijing Municipality[IDHT20180508] ; Beijing Natural Science Foundation[2162013] ; Beijing Natural Science Foundation[2172054] ; Construction of Scientific Research Platform[2018XK002]
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
Funding OrganizationProject of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges under Beijing Municipality ; Beijing Natural Science Foundation ; Construction of Scientific Research Platform
WOS IDWOS:000509816200042
PublisherELSEVIER
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Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/39286
Collection中国科学院过程工程研究所
Corresponding AuthorZhang, Rongyue
Affiliation1.Beijing Inst Petrochem Technol, Beijing Key Lab Fuels Cleaning & Adv Catalyt Emis, Coll Chem Engn, Beijing 102617, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
An, Ning,Gong, Pisheng,Hou, Henglei,et al. Fabrication of macroporous microspheres with core-shell structure for negative chromatography purification of virus[J]. JOURNAL OF CHROMATOGRAPHY A,2020,1610:4.
APA An, Ning.,Gong, Pisheng.,Hou, Henglei.,Chi, Weiya.,Jin, Haibo.,...&Zhang, Rongyue.(2020).Fabrication of macroporous microspheres with core-shell structure for negative chromatography purification of virus.JOURNAL OF CHROMATOGRAPHY A,1610,4.
MLA An, Ning,et al."Fabrication of macroporous microspheres with core-shell structure for negative chromatography purification of virus".JOURNAL OF CHROMATOGRAPHY A 1610(2020):4.
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