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Structural Changes and Antibacterial Activity of Epsilon-poly-L-lysine in Response to pH and Phase Transition and Their Mechanisms
Liu, Jia-Ning1,2; Chang, Sen-Lin1,2; Xu, Peng-Wei1,2; Tan, Ming-Hui1,2; Zhao, Bing1,2; Wang, Xiao-Dong1,2; Zhao, Qing-Sheng1,2
2020-01-29
Source PublicationJOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN0021-8561
Volume68Issue:4Pages:1101-1109
Abstractepsilon-Poly-L-lysine (epsilon-PL) consists of 25-35 lysine residues which are linked by an isopeptide bond formed by dehydration condensation of alpha-carboxyl and epsilon-amino groups and has good antibacterial activity and broad-spectrum inhibition range. However, there is no clear conclusion about the structure and antibacterial mechanism of epsilon-PL in aqueous solution. Herein, a high purity of epsilon-PL was prepared using Amberlite IRC-50 ion-exchange resin. Membrane filtration and dynamic light scattering were used to study the variations of epsilon-PL aggregation in aqueous solution with pH value. The conformational changes and antibacterial activities of epsilon-PL and carbamoylated epsilon-PL in different water environments were studied with circular dichroism (CD) and inhibition zone. The structural changes during the spray-drying process were determined by Fourier transform infrared spectroscopy. The results indicated that the side chain amino charge played a decisive role in the epsilon-PL conformation and aggregation. epsilon-PL exhibited the properties of a beta-sheet during spray drying from acidic liquids to solids. The cation enhanced the antibacterial activity of epsilon-PL but did not play a key role. Instead, the backbone of epsilon-PL might determine the mechanism of epsilon-PL antibacterial.
Keywordepsilon-poly-L-lysine conformation beta-sheet pH responsivity carbamoylated epsilon-PL antibacterial activity
DOI10.1021/acs.jafc.9b07524
Language英语
WOS KeywordANTIMICROBIAL MECHANISM ; ESCHERICHIA-COLI ; ALPHA-HELIX ; BETA-SHEET ; POLYLYSINE ; STREPTOMYCES ; POLY(EPSILON-L-LYSINE)
Funding ProjectJiangsu Yiming Biological Co., Ltd.
WOS Research AreaAgriculture ; Chemistry ; Food Science & Technology
WOS SubjectAgriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
Funding OrganizationJiangsu Yiming Biological Co., Ltd.
WOS IDWOS:000510529800018
PublisherAMER CHEMICAL SOC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/39363
Collection中国科学院过程工程研究所
Corresponding AuthorZhao, Qing-Sheng
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Beijing, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Liu, Jia-Ning,Chang, Sen-Lin,Xu, Peng-Wei,et al. Structural Changes and Antibacterial Activity of Epsilon-poly-L-lysine in Response to pH and Phase Transition and Their Mechanisms[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2020,68(4):1101-1109.
APA Liu, Jia-Ning.,Chang, Sen-Lin.,Xu, Peng-Wei.,Tan, Ming-Hui.,Zhao, Bing.,...&Zhao, Qing-Sheng.(2020).Structural Changes and Antibacterial Activity of Epsilon-poly-L-lysine in Response to pH and Phase Transition and Their Mechanisms.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,68(4),1101-1109.
MLA Liu, Jia-Ning,et al."Structural Changes and Antibacterial Activity of Epsilon-poly-L-lysine in Response to pH and Phase Transition and Their Mechanisms".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 68.4(2020):1101-1109.
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