CAS OpenIR
Quantitative profiling of integrin alpha v beta 3 on single cells with quantum dot labeling to reveal the phenotypic heterogeneity of glioblastoma
Wang, Tingting1,4; Li, Guang2; Wang, Dianbing1; Li, Feng3; Men, Dong3; Hu, Tao5; Xi, Yan1,3; Zhang, Xian-En1,4
2019-10-21
Source PublicationNANOSCALE
ISSN2040-3364
Volume11Issue:39Pages:18224-18231
AbstractThe distribution, localization and density of individual molecules (e.g. drug-specific receptors) on single cells can offer profound information about cell phenotypes. Profiling this information is a new research direction within the field of single cell biology, but it remains technically challenging. Through the combined use of quantum dot labeling, structured illumination microscopy (SIM) and computer-aided local surface reconstruction, we acquired a 3D imaging map of a drug target molecule, integrin alpha v beta 3, on glioblastoma cells at the single cell level. The results revealed that integrin alpha v beta 3 exhibits discrete distribution on the surface of glioblastoma cells, with its density differing significantly among cell lines. The density is illustrated as the approximate number of target molecules per mu m(2) on the irregular cell surface, ranging from 0 to 1.6. Functional studies revealed that the sensitivity of glioblastoma cells to inhibitor molecules depends on the density of the target molecules. After inhibitor treatment, the viability and invasion ability of different glioblastoma cells were highly correlated with the density of integrin alpha v beta 3 on their surfaces. This study not only provides a novel protocol for the quantitative analysis of surface proteins from irregular single cells, but also offers a clue for understanding the heterogeneity of tumor cells on the basis of molecular phenotypes. Thus, this work has potential significance in guiding targeted therapies for cancers.
DOI10.1039/c9nr01105f
Language英语
WOS KeywordEXPRESSION ; ALPHA(V)BETA(3) ; INFORMATICS
Funding ProjectChinese Academy of Sciences[XDB29050100] ; National Key Research and Development Program of China[2017YFA0205500]
WOS Research AreaChemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS SubjectChemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
Funding OrganizationChinese Academy of Sciences ; National Key Research and Development Program of China
WOS IDWOS:000512634500030
PublisherROYAL SOC CHEMISTRY
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/39368
Collection中国科学院过程工程研究所
Corresponding AuthorWang, Dianbing; Zhang, Xian-En
Affiliation1.Chinese Acad Sci, Natl Lab Biomacromol, CAS Ctr Excellence Biomacromol, Inst Biophys, Beijing 100101, Peoples R China
2.Huazhong Agr Univ, Coll Informat, Wuhan 430070, Peoples R China
3.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 4300071, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Wang, Tingting,Li, Guang,Wang, Dianbing,et al. Quantitative profiling of integrin alpha v beta 3 on single cells with quantum dot labeling to reveal the phenotypic heterogeneity of glioblastoma[J]. NANOSCALE,2019,11(39):18224-18231.
APA Wang, Tingting.,Li, Guang.,Wang, Dianbing.,Li, Feng.,Men, Dong.,...&Zhang, Xian-En.(2019).Quantitative profiling of integrin alpha v beta 3 on single cells with quantum dot labeling to reveal the phenotypic heterogeneity of glioblastoma.NANOSCALE,11(39),18224-18231.
MLA Wang, Tingting,et al."Quantitative profiling of integrin alpha v beta 3 on single cells with quantum dot labeling to reveal the phenotypic heterogeneity of glioblastoma".NANOSCALE 11.39(2019):18224-18231.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Tingting]'s Articles
[Li, Guang]'s Articles
[Wang, Dianbing]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Tingting]'s Articles
[Li, Guang]'s Articles
[Wang, Dianbing]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Tingting]'s Articles
[Li, Guang]'s Articles
[Wang, Dianbing]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.