CAS OpenIR
Comparative evaluation of the physicochemical properties of nano-hydroxyapatite/collagen and natural bone ceramic/collagen scaffolds and their osteogenesis-promoting effect on MC3T3-E1 cells
Lei, Xiongxin1; Gao, Jianping1,2; Xing, Fangyu3; Zhang, Yang4; Ma, Ye5; Zhang, Guifeng1,2
2019-12-01
Source PublicationREGENERATIVE BIOMATERIALS
ISSN2056-3418
Volume6Issue:6Pages:361-371
AbstractThe use of various types of calcium phosphate has been reported in the preparation of repairing materials for bone defects. However, the physicochemical and biological properties among them might be vastly different. In this study, we prepared two types of calcium phosphates, nano-hydroxyapatite (nHA) and natural bone ceramic (NBC), into 3D scaffolds by mixing with type I collagen (CoL), resulting in the nHA/CoL and NBC/CoL scaffolds. We then evaluated and compared the physicochemical and biological properties of these two calcium phosphates and their composite scaffold with CoL. Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD) and compressive tests were used to, respectively, characterize the morphology, composition, distribution and the effect of nHA and NBC to collagen. Next, we examined the biological properties of the scaffolds using cytotoxicity testing, flow cytometry, immunofluorescence staining, biocompatibility testing, CCK-8 assays and RT-PCR. The results reflected that the Ca2+ released from nHA and NBC could bind chemically with collagen and affect its physicochemical properties, including the infrared absorption spectrum and compression modulus, among others. Furthermore, the two kinds of scaffolds could promote the expression of osteo-relative genes, but showed different gene induction properties. In short, NBC/CoL could promote the expression of early osteogenic genes, while nHA/CoL could upregulate late osteogenic genes. Conclusively, these two composite scaffolds could provide MC3T3-E1 cells with a biomimetic surface for adhesion, proliferation and the formation of mineralized extracellular matrices. Moreover, nHA/CoL and NBC/CoL had different effects on the period and extent ofMC3T3-E1 cell mineralization.
Keywordnano-hydroxyapatite natural bone ceramic collagen scaffold MC3T3-E1 osteoblasts
DOI10.1093/rb/rbz026
Language英语
WOS KeywordSELF-ORGANIZATION MECHANISM ; CALCIUM-PHOSPHATE CEMENTS ; IN-VITRO ; DRUG-DELIVERY ; COLLAGEN ; SPECTROSCOPY ; COMPOSITES ; PARTICLES ; CERAMICS ; MATRICES
Funding ProjectNational Key Research and Development Program of China[2018YFA0108200] ; National Key Research and Development Program of China[2018YFC116400] ; International Cooperation Project of Hainan Province[KJHZ2015-30] ; Sci&Tech Program of Guangzhou of China[201803010086]
WOS Research AreaMaterials Science
WOS SubjectMaterials Science, Biomaterials
Funding OrganizationNational Key Research and Development Program of China ; International Cooperation Project of Hainan Province ; Sci&Tech Program of Guangzhou of China
WOS IDWOS:000517151900005
PublisherOXFORD UNIV PRESS
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/39435
Collection中国科学院过程工程研究所
Corresponding AuthorZhang, Guifeng
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Sch Chem & Engn, Beijing 100049, Peoples R China
3.Shandong Agr Univ, Coll Food Sci & Engn, Tai An 271018, Shandong, Peoples R China
4.Henan Inst Sci & Technol, Sch Life Sci & Technol, Xinxiang 453003, Henan, Peoples R China
5.Guandong Pharmaceut Univ, Sch Basic Course, Dept Pathogen Biol & Immunol, Guangzhou 510006, Peoples R China
Recommended Citation
GB/T 7714
Lei, Xiongxin,Gao, Jianping,Xing, Fangyu,et al. Comparative evaluation of the physicochemical properties of nano-hydroxyapatite/collagen and natural bone ceramic/collagen scaffolds and their osteogenesis-promoting effect on MC3T3-E1 cells[J]. REGENERATIVE BIOMATERIALS,2019,6(6):361-371.
APA Lei, Xiongxin,Gao, Jianping,Xing, Fangyu,Zhang, Yang,Ma, Ye,&Zhang, Guifeng.(2019).Comparative evaluation of the physicochemical properties of nano-hydroxyapatite/collagen and natural bone ceramic/collagen scaffolds and their osteogenesis-promoting effect on MC3T3-E1 cells.REGENERATIVE BIOMATERIALS,6(6),361-371.
MLA Lei, Xiongxin,et al."Comparative evaluation of the physicochemical properties of nano-hydroxyapatite/collagen and natural bone ceramic/collagen scaffolds and their osteogenesis-promoting effect on MC3T3-E1 cells".REGENERATIVE BIOMATERIALS 6.6(2019):361-371.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Lei, Xiongxin]'s Articles
[Gao, Jianping]'s Articles
[Xing, Fangyu]'s Articles
Baidu academic
Similar articles in Baidu academic
[Lei, Xiongxin]'s Articles
[Gao, Jianping]'s Articles
[Xing, Fangyu]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Lei, Xiongxin]'s Articles
[Gao, Jianping]'s Articles
[Xing, Fangyu]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.