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A homogeneous magnetic bead-based impedance immunosensor for highly sensitive detection of Escherichia coli O157:H7
Wang, Shujun1; Sun, Chongyun2; Hu, Qiushi1; Li, Shuang1; Wang, Chengbin3; Wang, Peng4; Zhou, Lei1
2020-04-15
Source PublicationBIOCHEMICAL ENGINEERING JOURNAL
ISSN1369-703X
Volume156Pages:6
Abstract

As foodborne pathogen contamination has become a threat to human health worldwide, early detection of pathogen is significant. Traditional electrochemical immunosensors for pathogen detection involve electrode modification or samples adsorption on the electrode which affect the detection sensitivity and electrode reproducibility, thereby limiting their further application in POCT (point of care testing). We established a homogeneous magnetic bead-based electrochemical impedance system using interdigitated gold electrodes as sensing elements for high sensitivity detection of Escherichia coli. (E. coli) O157:H7. The system avoided electrode modification and sample matrix adsorption, and greatly improved detection sensitivity. The detection limit of this method for E.coli O157:H7 was 10(2) colony-forming units per milliliter (CFU mL(-1)), coefficient of variation (CV) < 10 % (n = 3), which was three orders of magnitude lower than the traditional ELISA method, and the linearity detection range was 10(2) CFU mL(-1) to 10(6) CFU mL(-1). This magnetic bead-based impedance method is expected to be used for a wider range of pathogen targets analysis.

KeywordImpedance Immunosensor Magnetic Bead Escherichia Coli O157:H7
DOI10.1016/j.bej.2020.107513
Language英语
WOS KeywordUltrasensitive Electrochemical Immunoassay ; Rapid Detection ; Biosensors ; Nanomaterials ; Bacteria ; Cells ; Water ; Milk ; Food ; Tool
Funding ProjectBeijing Nova Program[Z151100000315086] ; Innovation Program of National Defense Science and Technology[17-163-12-ZT-005-029-01] ; National Key Research and Development Program[2016YFC1202502]
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
Funding OrganizationBeijing Nova Program ; Innovation Program of National Defense Science and Technology ; National Key Research and Development Program
WOS IDWOS:000518151700011
PublisherELSEVIER
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/39483
Collection中国科学院过程工程研究所
Corresponding AuthorWang, Chengbin; Wang, Peng; Zhou, Lei
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, PLA Key Lab Biopharmaceut Prod & Formulat Engn, Beijing 100190, Peoples R China
2.Beijing Red Cross Blood Ctr, Blood Grouping Lab, Beijing 100088, Peoples R China
3.Chinese Peoples Liberat Army Gen Hosp, Dept Clin Lab, Beijing 100853, Peoples R China
4.Tsinghua Univ, Dept Precis Instrument, State Key Lab Precis Measurements, Beijing 100084, Peoples R China
Recommended Citation
GB/T 7714
Wang, Shujun,Sun, Chongyun,Hu, Qiushi,et al. A homogeneous magnetic bead-based impedance immunosensor for highly sensitive detection of Escherichia coli O157:H7[J]. BIOCHEMICAL ENGINEERING JOURNAL,2020,156:6.
APA Wang, Shujun.,Sun, Chongyun.,Hu, Qiushi.,Li, Shuang.,Wang, Chengbin.,...&Zhou, Lei.(2020).A homogeneous magnetic bead-based impedance immunosensor for highly sensitive detection of Escherichia coli O157:H7.BIOCHEMICAL ENGINEERING JOURNAL,156,6.
MLA Wang, Shujun,et al."A homogeneous magnetic bead-based impedance immunosensor for highly sensitive detection of Escherichia coli O157:H7".BIOCHEMICAL ENGINEERING JOURNAL 156(2020):6.
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