Knowledge Management System Of Institute of process engineering,CAS
| Efficient enzymatic synthesis of cephalexin in suspension aqueous solution system | |
| Fan, Yixiao1,2; Li, Yingbo1; Liu, Qingfen1 | |
| 2020-03-02 | |
| Source Publication | BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
 |
| ISSN | 0885-4513 |
| Pages | 13 |
| Abstract | An efficient method for the enzymatic synthesis of cephalexin (CEX) from 7-amino-3-deacetoxycephalosporanic acid (7-ADCA) and d-phenylglycine methyl ester (PGME) using immobilized penicillin G acylase (IPGA) as catalyst in a suspension aqueous solution system was developed, where the reactant 7-ADCA and product CEX are mainly present as solid particles. The effects of key factors on the enzymatic synthesis were investigated. Results showed that continuous feeding of PGME was more efficient for the synthesis of CEX than the batch mode. Under the optimized conditions, the maximum 7-ADCA conversion ratio of 99.3% and productivity of 200 mmol/L/H were achieved, both of which are much superior to the homogeneous aqueous solution system. Besides, IPGA still retained 95.4% of its initial activity after 10 cycles of enzymatic synthesis, indicating the excellent stability of this approach. The developed approach shows great potential for the industrial production of CEX via an enzyme-based route. |
| Keyword | cephalexin enzymatic synthesis penicillin G acylase suspension aqueous solution system beta-lactam antibiotics |
| DOI | 10.1002/bab.1903 |
| Language | 英语 |
| WOS Keyword | IMMOBILIZED PENICILLIN ACYLASE ; BETA-LACTAM ANTIBIOTICS ; KINETICALLY CONTROLLED SYNTHESIS ; HIGH SUBSTRATE CONCENTRATIONS ; SOLID PEPTIDE-SYNTHESIS ; ETHYLENE-GLYCOL MEDIUM ; SITU PRODUCT REMOVAL ; INDUSTRIAL-PRODUCTION ; CATALYZED SYNTHESIS ; ORGANIC MEDIUM |
| Funding Project | Major Science and Technology Project of Water Pollution Control and Management in China[2017ZX07402003] ; Key Research Program of the Chinese Academy of Sciences[ZDRW-ZS-2016-5-3] ; National Natural Science Foundation of China[21676272] |
| WOS Research Area | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS Subject | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| Funding Organization | Major Science and Technology Project of Water Pollution Control and Management in China ; Key Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China |
| WOS ID | WOS:000517500000001 |
| Publisher | WILEY |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/39525 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Li, Yingbo; Liu, Qingfen |
| Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, CAS Key Lab Green Proc & Engn, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Sch Chem Engn, Beijing, Peoples R China |
| First Author Affilication | Center of lonic Liquids and Green Engineering |
| Corresponding Author Affilication | Center of lonic Liquids and Green Engineering |
| Recommended Citation GB/T 7714 | Fan, Yixiao,Li, Yingbo,Liu, Qingfen. Efficient enzymatic synthesis of cephalexin in suspension aqueous solution system[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2020:13. |
| APA | Fan, Yixiao,Li, Yingbo,&Liu, Qingfen.(2020).Efficient enzymatic synthesis of cephalexin in suspension aqueous solution system.BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,13. |
| MLA | Fan, Yixiao,et al."Efficient enzymatic synthesis of cephalexin in suspension aqueous solution system".BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY (2020):13. |
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