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HBc-based virus-like particle assembly from inclusion bodies using 2-methyl-2, 4-pentanediol
Zhang, Yao1,2; Yin, Shuang1,3; Zhang, Bingyang1,3; Bi, Jingxiu3; Liu, Yongdong1; Su, Zhiguo1
2020-02-01
Source PublicationPROCESS BIOCHEMISTRY
ISSN1359-5113
Volume89Pages:233-237
AbstractHepatitis B virus core antigen (HBc) has recently been used as carriers to develop recombinant vaccines. However, not virus-like particles (VLPs) but inactive inclusion bodies are often formed for the chimeric proteins when expressed in Escherichia coli. A novel method for in vitro assembly of chimeric HBc-MAGE3 II from inclusion bodies to VLPs was established in this study. The method utilized 2-methyl-2, 4-pentanediol (MPD), an amphipathic di-alcohol, to dissociate sodium dodecyl sulfate (SDS) from the solubilized chimeric protein to initiate VLP assembly. The HBc-MAGE3 II could assemble into VLPs only when the molar ratio of SDS/protein subunit was less than 0.14. After removing SDS/MPD by desalting and further purification, VLPs with similar morphology to the natural virus were obtained. This method could be used for preparation of other VLPs expressed as inclusion bodies.
KeywordVirus-like particle (VLP) Hepatitis B virus core antigen (HBc) Inclusion body Assembly Sodium dodecyl sulfate (SDS) 2-methyl-2, 4-pentanediol (MPD)
DOI10.1016/j.procbio.2019.10.031
Language英语
WOS KeywordSODIUM DODECYL-SULFATE ; SDS ; DETERGENTS ; PROTEINS ; REMOVAL ; PURIFICATION ; LYSOZYME ; EXCHANGE ; BINDING
Funding ProjectNational Natural Science Foundation of China[21576267]
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
Funding OrganizationNational Natural Science Foundation of China
WOS IDWOS:000517856100025
PublisherELSEVIER SCI LTD
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Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/39583
Collection中国科学院过程工程研究所
Corresponding AuthorLiu, Yongdong; Su, Zhiguo
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Univ Adelaide, Sch Chem Engn & Adv Mat, Adelaide, SA 5005, Australia
Recommended Citation
GB/T 7714
Zhang, Yao,Yin, Shuang,Zhang, Bingyang,et al. HBc-based virus-like particle assembly from inclusion bodies using 2-methyl-2, 4-pentanediol[J]. PROCESS BIOCHEMISTRY,2020,89:233-237.
APA Zhang, Yao,Yin, Shuang,Zhang, Bingyang,Bi, Jingxiu,Liu, Yongdong,&Su, Zhiguo.(2020).HBc-based virus-like particle assembly from inclusion bodies using 2-methyl-2, 4-pentanediol.PROCESS BIOCHEMISTRY,89,233-237.
MLA Zhang, Yao,et al."HBc-based virus-like particle assembly from inclusion bodies using 2-methyl-2, 4-pentanediol".PROCESS BIOCHEMISTRY 89(2020):233-237.
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