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| Immunization with a fusion protein vaccine candidate generated from truncated peptides of human enterovirus 71 protects mice from lethal enterovirus 71 infections | |
| Liu,Jiangning1; Zhao,Binbin1; Xue,Ling2,3; Wu,Jing1; Xu,Yanfeng1; Liu,Yongdong2,3; Qin,Chuan1 | |
| 2020-04-22 | |
| Source Publication | Virology Journal
 |
| Volume | 17Issue:1 |
| Abstract | AbstractBackgroundProphylactic vaccines are critical in preventing hand, foot, and mouth disease (HFMD) primarily caused by human enterovirus 71 (EV71) infection. Children aged less than 5?years are especially susceptible to EV71 infections. In addition to the development of vaccines containing the inactivated virus, those containing virus-like particles (VLPs) with repeated antigens also constitute an effective preventive strategy for EV71 infections, with safety and productivity advantages. We previously developed a fusion protein composed with truncated peptides of the EV71 capsid protein, which assembled into spherical particles. This study aimed to assess the immunoprotective effects of this fusion protein as a vaccine candidate in a mouse model of EV71 infection.MethodsTo evaluate the protective effect of fusion protein vaccine candidate, neonatal mice born by immunized female mice, as well as normal neonatal mice immunized twice were infected with EV71 virus. Whereafter, the survival rates, clinical scores and viral loads were measured.ResultsThe high dosage and booster immunization helped induce specific serum antibodies with high neutralization titers, which were transferred to neonatal mice, thereby facilitating effective resistance towards EV71 infection. An active immune response was also observed in neonatal mice which generated following immunization.ConclusionsThe present results suggest that this fusion protein is a suitable vaccine candidate in treating EV71 infections. |
| Keyword | Human Enterovirus 71 Vaccine candidate Fusion protein Mouse model Infection |
| DOI | 10.1186/s12985-020-01328-8 |
| Language | 英语 |
| WOS ID | BMC:10.1186/s12985-020-01328-8 |
| Publisher | BioMed Central |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/39695 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Liu,Yongdong; Qin,Chuan |
| Affiliation | 1.NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases, Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Institute of Laboratory Animal Science, CAMS&PUMC 2.National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences 3.University of Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Liu,Jiangning,Zhao,Binbin,Xue,Ling,et al. Immunization with a fusion protein vaccine candidate generated from truncated peptides of human enterovirus 71 protects mice from lethal enterovirus 71 infections[J]. Virology Journal,2020,17(1). |
| APA | Liu,Jiangning.,Zhao,Binbin.,Xue,Ling.,Wu,Jing.,Xu,Yanfeng.,...&Qin,Chuan.(2020).Immunization with a fusion protein vaccine candidate generated from truncated peptides of human enterovirus 71 protects mice from lethal enterovirus 71 infections.Virology Journal,17(1). |
| MLA | Liu,Jiangning,et al."Immunization with a fusion protein vaccine candidate generated from truncated peptides of human enterovirus 71 protects mice from lethal enterovirus 71 infections".Virology Journal 17.1(2020). |
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