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Succinate Production with Metabolically Engineered Escherichia coli Using Elephant Grass Stalk (Pennisetum purpureum) Hydrolysate as Carbon Source
Olajuyin, Ayobami Matthew1,2; Yang, Maohua1,2; Mu, Tingzhen1,2; Sharshar, Moustafa Mohamed1,2; Xing, Jianmin1,2
2020-05-01
Source PublicationWASTE AND BIOMASS VALORIZATION
ISSN1877-2641
Volume11Issue:5Pages:1717-1725
AbstractSuccinic acid is a spectacular chemical that can be used as the precursor of various industrial products including pharmaceuticals and biochemicals. The improvement of the succinic acid market depends on strains engineering that is capable of producing succinic acid at high yield and excellent growth rate which could utilize the wide range of carbon sources such as renewable biomass. Here we use counter selection using catAsacB for pathway design and strains developments. In this investigation, metabolically engineered Escherichia coli M6PM strain was constructed for the synthesis of succinic acid using elephant grass stalk (Pennisetum purpureum) as a carbon source. Elephant grass stalk hydrolysate was prepared which comprised of 11.60 +/- 0.04 g/L glucose, 27.22 +/- 0.04 g/L xylose and 0.65 +/- 0.04 g/L arabinose. Metabolically engineered E. coli M6PM was constructed and fermentation with pure sugars revealed that it could utilize xylose and glucose efficiently. E. coli M6PM produced a final succinate concentration of 30.03 +/- 0.02 g/L and a yield of 1.09 mol/mol during 72 h dual-phase fermentation using elephant grass stalk hydrolysate, which resulted in 64% maximum theoretical yield of succinic acid. The high succinate yield from elephant grass stalk demonstrated possible application of renewable biomass as feedstock for the synthesis of succinic acid using recombinant E. coli.
KeywordEscherichia coli Succinic acid Elephant grass stalk Dual-phase fermentation
DOI10.1007/s12649-018-0524-2
Language英语
WOS KeywordEFFICIENT PRODUCTION ; ACID PRODUCTION ; PATHWAYS ; GROWTH ; ISOMERASE ; STRAINS ; YIELD ; STATE
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000523383800008
PublisherSPRINGER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/39953
Collection中国科学院过程工程研究所
Corresponding AuthorXing, Jianmin
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Olajuyin, Ayobami Matthew,Yang, Maohua,Mu, Tingzhen,et al. Succinate Production with Metabolically Engineered Escherichia coli Using Elephant Grass Stalk (Pennisetum purpureum) Hydrolysate as Carbon Source[J]. WASTE AND BIOMASS VALORIZATION,2020,11(5):1717-1725.
APA Olajuyin, Ayobami Matthew,Yang, Maohua,Mu, Tingzhen,Sharshar, Moustafa Mohamed,&Xing, Jianmin.(2020).Succinate Production with Metabolically Engineered Escherichia coli Using Elephant Grass Stalk (Pennisetum purpureum) Hydrolysate as Carbon Source.WASTE AND BIOMASS VALORIZATION,11(5),1717-1725.
MLA Olajuyin, Ayobami Matthew,et al."Succinate Production with Metabolically Engineered Escherichia coli Using Elephant Grass Stalk (Pennisetum purpureum) Hydrolysate as Carbon Source".WASTE AND BIOMASS VALORIZATION 11.5(2020):1717-1725.
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