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| In-situ and sensitive stability study of emulsion and aluminum adjuvanted inactivated foot-and-mouth disease virus vaccine by differential scanning fluorimetry analysis | |
| Song, Yanmin1,2; Yang, Yanli1; Lin, Xuan1,2; Li, Xiunan1; Zhang, Xuan1; Ma, Guanghui1; Su, Zhiguo1; Zhang, Songping1 | |
| 2020-03-23 | |
| Source Publication | VACCINE
 |
| ISSN | 0264-410X |
| Volume | 38Issue:14Pages:2904-2912 |
| Abstract | Adjuvants are important to enhance the antigens immunogenicity, but may also alter the structures of antigens. Currently off-line methods for adjuvants induced antigen alteration suffer from incomplete release and possible structural alteration of antigens. Here we investigated the differential scanning fluorimetry (DSF) as an in-situ and high-throughput strategy to analyze the stability of inactivated foot-and- mouth disease virus (iFMDV), known as 146S, in three representative adjuvants including aluminum hydroxide (AH), oil-in-water (O/W) emulsion, and water-in-oil (W/O) emulsion. Under optimized DSF conditions, the T-m referring to 146S dissociation can be detected in all three adjuvants. Using SYBR Green II as fluorescent dye enables detection of iFMDV as low as 5 mu g/mL. By comparing the T-m in different pH, three adjuvants showed different effects on 146S. Screening for excipients was successfully conducted using DSF. Sugars and glycerol increased the T-m of iFMDV in all three adjuvants, but to different degree. The stabilization by 20% (w/v) sucrose and glycerol was further verified by differential scanning calorimetry (DSC) and high performance size exclusion chromatography (HPSEC). DSF is proved also applicative for low-purity iFMDV and pre-adjuvanted iFMDV vaccines. In summary, the DSF can be a powerful tool in formulation study and vaccine quality control for inactivated virus vaccines. (C) 2020 Elsevier Ltd. All rights reserved. |
| Keyword | DSF In-situ Adjuvant iFMDV Stability High throughput |
| DOI | 10.1016/j.vaccine.2020.02.068 |
| Language | 英语 |
| WOS Keyword | SIZE-EXCLUSION HPLC ; B SURFACE-ANTIGEN ; QUALITY-CONTROL ; ADSORPTION ; IMMUNOGENICITY ; FORMULATION ; PARTICLES ; THERMOSTABILITY ; STABILIZATION ; DENATURATION |
| Funding Project | National Natural Sciences Foundation of China[21808226] ; National Natural Sciences Foundation of China[21821005] ; National Natural Sciences Foundation of China[31970872] |
| WOS Research Area | Immunology ; Research & Experimental Medicine |
| WOS Subject | Immunology ; Medicine, Research & Experimental |
| Funding Organization | National Natural Sciences Foundation of China |
| WOS ID | WOS:000525317400003 |
| Publisher | ELSEVIER SCI LTD |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/40044 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Su, Zhiguo; Zhang, Songping |
| Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Sch Chem Engn, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Song, Yanmin,Yang, Yanli,Lin, Xuan,et al. In-situ and sensitive stability study of emulsion and aluminum adjuvanted inactivated foot-and-mouth disease virus vaccine by differential scanning fluorimetry analysis[J]. VACCINE,2020,38(14):2904-2912. |
| APA | Song, Yanmin.,Yang, Yanli.,Lin, Xuan.,Li, Xiunan.,Zhang, Xuan.,...&Zhang, Songping.(2020).In-situ and sensitive stability study of emulsion and aluminum adjuvanted inactivated foot-and-mouth disease virus vaccine by differential scanning fluorimetry analysis.VACCINE,38(14),2904-2912. |
| MLA | Song, Yanmin,et al."In-situ and sensitive stability study of emulsion and aluminum adjuvanted inactivated foot-and-mouth disease virus vaccine by differential scanning fluorimetry analysis".VACCINE 38.14(2020):2904-2912. |
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