CAS OpenIR
Fabrication of a Cartilage Patch by Fusing Hydrogel-Derived Cell Aggregates Onto Electrospun Film
Zhang, Jiabin1; Yun, Seonho1; Du, Yuguang2; Zannettino, Andrew C. W.3; Zhang, Hu1,4
2020-03-19
Source PublicationTISSUE ENGINEERING PART A
ISSN1937-3341
Pages9
AbstractIrregular defects at sites of degenerative cartilage often accompany osteoarthritis (OA). The development of novel cell-/biomaterial-based cartilage tissue engineering methods to address these defects may provide a durable approach to hinder the development of OA. In this study, we fabricated a neocartilage patch by fusing cell aggregates onto a biodegradable nanofiber film for degenerative cartilage repair. Human mesenchymal stem/stromal cell (MSC) aggregates were prepared and induced for chondrogenesis in a thermosensitive hydrogel, poly (N-isopropylacrylamide-co-acrylic acid (p(NIPAAm-AA)). Cell migration mediated the formation of cell aggregates in the thermosensitive hydrogel and led to a cell-dense hollow shell structure. The chondrocytes derived from MSC aggregates in the hydrogel were evidenced by the expression of chondrogenesis-related genes and extracellular matrices. They were fused onto an electrospun film by mechanical force and spatial confinement to generate a neo-cartilage patch. The fabricated neocartilage patches may be able to integrate into the irregular defects under compressive stresses and achieve cartilage regeneration in vivo. Impact statement The formation of human mesenchymal stem/stromal cells aggregates in thermosensitive hydrogels was mechanistically examined. These in situ formed cell aggregates with enhanced chondrogenesis were bioengineered into a neocartilage patch for regeneration of superficial irregular cartilage defects.
Keywordhydrogel chondrogenesis human mesenchymal stem stromal cell electrospinning cell aggregates cartilage regeneration
DOI10.1089/ten.tea.2019.0318
Language英语
WOS KeywordSTEM-CELLS ; ARTICULAR-CARTILAGE ; TISSUE ; CULTURE ; REPAIR ; DIFFERENTIATION ; MIGRATION
Funding ProjectAustralian Research Council Discovery Project[DP160104632] ; Medical Advancement Without Animal (MAWA) Trust
WOS Research AreaCell Biology ; Engineering ; Materials Science
WOS SubjectCell & Tissue Engineering ; Cell Biology ; Engineering, Biomedical ; Materials Science, Biomaterials
Funding OrganizationAustralian Research Council Discovery Project ; Medical Advancement Without Animal (MAWA) Trust
WOS IDWOS:000525891200001
PublisherMARY ANN LIEBERT, INC
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/40086
Collection中国科学院过程工程研究所
Corresponding AuthorZannettino, Andrew C. W.; Zhang, Hu
Affiliation1.Univ Adelaide, Sch Chem Engn & Adv Mat, Adelaide, SA, Australia
2.Chinese Acad Sci, Inst Proc Engn, Beijing, Peoples R China
3.Univ Adelaide, Adelaide Med Sch, Adelaide, SA 5001, Australia
4.Keck Grad Inst, Henry E Riggs Sch Appl Life Sci, Claremont, CA 91711 USA
Recommended Citation
GB/T 7714
Zhang, Jiabin,Yun, Seonho,Du, Yuguang,et al. Fabrication of a Cartilage Patch by Fusing Hydrogel-Derived Cell Aggregates Onto Electrospun Film[J]. TISSUE ENGINEERING PART A,2020:9.
APA Zhang, Jiabin,Yun, Seonho,Du, Yuguang,Zannettino, Andrew C. W.,&Zhang, Hu.(2020).Fabrication of a Cartilage Patch by Fusing Hydrogel-Derived Cell Aggregates Onto Electrospun Film.TISSUE ENGINEERING PART A,9.
MLA Zhang, Jiabin,et al."Fabrication of a Cartilage Patch by Fusing Hydrogel-Derived Cell Aggregates Onto Electrospun Film".TISSUE ENGINEERING PART A (2020):9.
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