CAS OpenIR
Establishment and Application of Peristaltic Human Gut-Vessel Microsystem for Studying Host-Microbial Interaction
Jing, Bolin1,2; Wang, Zhuo A.1; Zhang, Chen1; Deng, Quanfeng3; Wei, Jinhua1; Luo, Yong3; Zhang, Xiuli4; Li, Jianjun1; Du, Yuguang1
2020-03-31
Source PublicationFRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
ISSN2296-4185
Volume8Pages:14
AbstractIntestinal floras influence a lot of biological functions of the organism. Although animal model are strong tools for researches on the relationship between host and microbe, a physiologically relevant in vitro human gut model was still required. Here, a novel human gut-vessel microfluidic system was established to study the host-microbial interaction. Peristaltic motion of the cells on the chip was driven by a pneumatic pump. When intestinal epithelial cells (Caco2) were co-cultured with vascular endothelial cells (HUVECs) on the peristaltic microfluidic chip, Caco2 showed normal barrier and absorption functions after 5 days cultivation, which generally took 21 days in static Transwell models. Intestinal microvilli and glycocalyx layer were seen after 4 days cultivation, and Lactobacillus casei was successfully co-cultured for a week in the intestinal cavity. A model for intestinal damage and inflammatory responses caused by E. coli was set up on this chip, which were successfully suppressed by Lactobacillus casei or antibiotic. In summary, this human gut-vessel microfluidic system showed a good potential for investigating the host-microbial interaction and the effect and mechanism of microbiome on intestinal diseases in vitro.
Keywordperistaltic microfluidic chip human gut-vessel co-culture model host-microbial interaction Lactobacillus casei enteritis injury
DOI10.3389/fbioe.2020.00272
Language英语
WOS KeywordINFLAMMATORY-BOWEL-DISEASE ; DRUG PERMEABILITY ; ENDOTHELIAL-CELLS ; STEM-CELLS ; CACO-2 ; LACTOBACILLI ; MODEL ; DIFFERENTIATION ; BIFIDOBACTERIA ; BACTERIA
Funding ProjectNational Natural Science Foundation of China[31870802] ; National Natural Science Foundation of China[31700705]
WOS Research AreaBiotechnology & Applied Microbiology ; Science & Technology - Other Topics
WOS SubjectBiotechnology & Applied Microbiology ; Multidisciplinary Sciences
Funding OrganizationNational Natural Science Foundation of China
WOS IDWOS:000526743400001
PublisherFRONTIERS MEDIA SA
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/40111
Collection中国科学院过程工程研究所
Corresponding AuthorZhang, Xiuli; Li, Jianjun; Du, Yuguang
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing, Peoples R China
2.Univ Chinese Acad Sci, Dept Chem, Beijing, Peoples R China
3.Dalian Univ Technol, Dept Chem Engn, Key Lab Fine Chem, Dalian, Peoples R China
4.Soochow Univ, Coll Pharmaceut Sci, Suzhou, Peoples R China
Recommended Citation
GB/T 7714
Jing, Bolin,Wang, Zhuo A.,Zhang, Chen,et al. Establishment and Application of Peristaltic Human Gut-Vessel Microsystem for Studying Host-Microbial Interaction[J]. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,2020,8:14.
APA Jing, Bolin.,Wang, Zhuo A..,Zhang, Chen.,Deng, Quanfeng.,Wei, Jinhua.,...&Du, Yuguang.(2020).Establishment and Application of Peristaltic Human Gut-Vessel Microsystem for Studying Host-Microbial Interaction.FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,8,14.
MLA Jing, Bolin,et al."Establishment and Application of Peristaltic Human Gut-Vessel Microsystem for Studying Host-Microbial Interaction".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 8(2020):14.
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