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| Overexpression and Biochemical Characterization of an Endo-alpha-1,4-polygalacturonase from Aspergillus nidulans in Pichia pastoris | |
| Xu, Hua1; Zhang, Pengfei2; Zhang, Yuchen3; Liu, Zebin4; Zhang, Xuebing3; Li, Zhimin1; Li, Jian-Jun3; Du, Yuguang3 | |
| 2020-03-01 | |
| Source Publication | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| Volume | 21Issue:6Pages:12 |
| Abstract | Pectinases have many applications in the industry of food, paper, and textiles, therefore finding novel polygalacturonases is required. Multiple sequence alignment and phylogenetic analysis of AnEPG (an endo-alpha-1,4-polygalacturonase from Aspergillus nidulans) and other GH 28 endo-polygalacturonases suggested that AnEPG is different from others. AnEPG overexpressed in Pichia pastoris was characterized. AnEPG showed the highest activity at pH 4.0, and exhibited moderate activity over a narrow pH range (pH 2.0-5.0) and superior stability in a wide pH range (pH 2.0-12.0). It displayed the highest activity at 60 degrees C, and retained >42.2% of maximum activity between 20 and 80 degrees C. It was stable below 40 degrees C and lost activity very quickly above 50 degrees C. Its apparent kinetic parameters against PGA (polygalacturonic acid) were determined, with the K-m and k(cat) values of 8.3 mg/mL and 5640 mu mol/min/mg, respectively. Ba2+ and Ni2+ enhanced activity by 12.2% and 9.4%, respectively, while Ca2+, Cu2+, and Mn2+ inhibited activity by 14.8%, 12.8%, and 10.2% separately. Analysis of hydrolysis products by AnEPG proved that AnEPG belongs to an endo-polygalacturonase. Modelled structure of AnEPG by I-TASSER showed structural characteristics of endo-polygalacturonases. This pectinase has great potential to be used in food industry and as feed additives. |
| Keyword | endo-alpha-1,4-polygalacturonase Aspergillus nidulans pH and temperature-rate profile pH and thermal stability identification of hydrolysis products structure modelling |
| DOI | 10.3390/ijms21062100 |
| Language | 英语 |
| WOS Keyword | ACIDIC ENDO-POLYGALACTURONASE ; POTENTIAL APPLICATION ; CRYSTAL-STRUCTURE ; EXPRESSION ; NIGER ; ENDOPOLYGALACTURONASE ; IDENTIFICATION ; PURIFICATION ; ENZYMES ; PECTIN |
| Funding Project | National Key Research and Development Program of China[2017YFD0200902] ; National Natural Science Foundation of China[31370799] |
| WOS Research Area | Biochemistry & Molecular Biology ; Chemistry |
| WOS Subject | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| Funding Organization | National Key Research and Development Program of China ; National Natural Science Foundation of China |
| WOS ID | WOS:000529890200196 |
| Publisher | MDPI |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/40576 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Li, Zhimin; Li, Jian-Jun |
| Affiliation | 1.Jiangxi Agr Univ, Coll Biosci & Bioengn, Nanchang 330045, Jiangxi, Peoples R China 2.Sichuan Normal Univ, Coll Life Sci, Chengdu 610101, Peoples R China 3.Chinese Acad Sci, Natl Engn Res Ctr Biotechnol Beijing, Key Lab Biopharmaceut Prod & Formulat Engn, Natl Key Lab Biochem Engn,PLA,Inst Proc Engn, Beijing 100190, Peoples R China 4.Capital Normal Univ, Coll Life Sci, Beijing 10048, Peoples R China |
| Recommended Citation GB/T 7714 | Xu, Hua,Zhang, Pengfei,Zhang, Yuchen,et al. Overexpression and Biochemical Characterization of an Endo-alpha-1,4-polygalacturonase from Aspergillus nidulans in Pichia pastoris[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2020,21(6):12. |
| APA | Xu, Hua.,Zhang, Pengfei.,Zhang, Yuchen.,Liu, Zebin.,Zhang, Xuebing.,...&Du, Yuguang.(2020).Overexpression and Biochemical Characterization of an Endo-alpha-1,4-polygalacturonase from Aspergillus nidulans in Pichia pastoris.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,21(6),12. |
| MLA | Xu, Hua,et al."Overexpression and Biochemical Characterization of an Endo-alpha-1,4-polygalacturonase from Aspergillus nidulans in Pichia pastoris".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21.6(2020):12. |
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