CAS OpenIR
高效液相色谱质谱法测定牛型胶原含量
邢芳毓1; 高建萍2; 张贵锋2; 王明林1
2020
Source Publication分析测试学报
ISSN1004-4957
Volume39Issue:4Pages:479
Abstract建立了一种基于高效液相色谱-质谱联用技术并通过特征多肽进行牛Ⅰ型胶原含量检测的方法。胶原海绵样品经酶解处理后进行色谱分离,在电喷雾离子源正离子扫描及保留时间依赖的单离子监测模式(SIM)下测定,外标法定量。采用胰蛋白酶特异性酶解得到牛Ⅰ型胶原的1个特征多肽为GFSGLDGAK。该多肽在0.645 ~ 21.5 μg /mL质量浓度范围内线性关系良好(r~2 = 0.999 4);方法的定量下限为6.45 × 10 ~(- 4) μg / mL;在3.10、2.48、1.55 μg /mL 3个加标水平下,平均回收率为98.2%~ 102%;峰面积的相对标准偏差(n = 6)为0.80%。胶原海绵样品经均匀性、稳定性和定值检测,确保了方法有效性,验证了该产品质量,且测得牛Ⅰ型胶原含量为(0.926 ± 0.014) mg /mg。该方法简便快速,重现性好、稳定性高,可有效地对牛Ⅰ型胶原进行定量。
Keywordhigh performance liquid chromatography - mass spectrometry (HPLC - MS) marker peptide bovine typeⅠ collagen quantitation 高效液相色谱- 质谱(HPLC - MS) 特征肽 牛Ⅰ型胶原 定量
DOI10.3969/j.issn.1004-4957.2020.04.008
Language英语
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/40597
Collection中国科学院过程工程研究所
Affiliation1.山东农业大学
2.中国科学院过程工程研究所
Recommended Citation
GB/T 7714
邢芳毓,高建萍,张贵锋,等. 高效液相色谱质谱法测定牛型胶原含量[J]. 分析测试学报,2020,39(4):479.
APA 邢芳毓,高建萍,张贵锋,&王明林.(2020).高效液相色谱质谱法测定牛型胶原含量.分析测试学报,39(4),479.
MLA 邢芳毓,et al."高效液相色谱质谱法测定牛型胶原含量".分析测试学报 39.4(2020):479.
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