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Allogeneic primary mesenchymal stem/stromal cell aggregates within poly(N-isopropylacrylamide-co-acrylic acid) hydrogel for osteochondral regeneration
Zhang, Jiabin1; Zhang, Ming3; Lin, Rongcai3; Yun, Seonho1; Du, Yuguang5; Wang, Liming3; Yao, Qingqiang3; Zannettino, Andrew2; Zhang, Hu1,4
2020-03-01
Source PublicationAPPLIED MATERIALS TODAY
ISSN2352-9407
Volume18Pages:16
Abstract

Osteoarthritis (OA) is an inflammation-related chronic disease that causes progressive degeneration of cartilage, which might even extend to subchondral bones. Due to the unique physiological structural differences between cartilage and subchondral bone, it is challenging to restore the full function of an osteochondral defect. In this study, a thermosensitive poly(N-isopropylacrylamide-co-acrylic acid) (p(NIPAAm-AA)) hydrogel was used as a carrier for allogeneic primary mesenchymal stem/stromal cells to determine the therapeutic efficacy of the cell-hydrogel hybrid on osteochondral regeneration. At a similar mechanical strength, p(NIPAAm-AA) hydrogel facilitates formation of cell aggregates of allogeneic primary MSCs in situ, while the fibrin hydrogel supports cell binding and the development of a spindle morphology. While the p(NIPAAm-AA) hydrogel did not induce any inflammatory effects, the fibrin hydrogel was found to elicit a pro-inflammatory response. MSC aggregates promotes expression of paracrine signaling-related genes (TGFB1, VEGF, CXCL12, IGF1, BMP2, BM137, WNT3A, CTNNB1) as well as expression of chondrogenesis-related genes (S0X9, ACAN, and COL2A1) and generation of extracellular matrices (ECMs) (type 2 collagen and glycosaminoglycans) in chondrogenic induction medium. The functional enhancement of paracrine effects by MSC aggregates (i.e. the immunomodulatory effect and the stimulation of stem/progenitor cells homing) and induced differentiation of MSCs might play a synergistic role in the generation of the neo-cartilage and subchondral bone at an osteochondral defect site in vivo. Delivery of primary allogeneic MSCs in the context of a thermosensitive p(NIPAAm-AA) hydrogel to an osteochondral defect site may be a novel and promising strategy for osteochondral regeneration. (C) 2019 Elsevier Ltd. All rights reserved.

KeywordThermosensitive Hydrogel Mesenchymal Stem/stromal Cells Cell Aggregates Osteochondral Regeneration
DOI10.1016/j.apmt.2019.100487
Language英语
WOS KeywordControlled-release ; Cartilage Repair ; Stromal Cells ; Stem-cells ; Bone ; Strategies ; Delivery ; Differentiation ; Matrix ; Biomaterials
Funding ProjectNational Key Research and Development Program of China[2018YFC1105200-4] ; ARC DiscoveryProject[DP160104632] ; Medical Advancement Without Animal (MAWA) Trust ; IPE-UoA Scholarship (BES)
WOS Research AreaMaterials Science
WOS SubjectMaterials Science, Multidisciplinary
Funding OrganizationNational Key Research and Development Program of China ; ARC DiscoveryProject ; Medical Advancement Without Animal (MAWA) Trust ; IPE-UoA Scholarship (BES)
WOS IDWOS:000530652300007
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/40824
Collection中国科学院过程工程研究所
Corresponding AuthorYao, Qingqiang; Zannettino, Andrew; Zhang, Hu
Affiliation1.Univ Adelaide, Sch Chem Engn, Adelaide, SA 5005, Australia
2.Univ Adelaide, Adelaide Med Sch, Adelaide, SA 5001, Australia
3.Nanjing Med Univ, Nanjing First Hosp, Inst Digital Med, Dept Orthopaed Surg, Nanjing 210006, Peoples R China
4.Keck Grad Inst, Henry E Riggs Sch Appl Life Sci, Claremont, CA 91711 USA
5.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Jiabin,Zhang, Ming,Lin, Rongcai,et al. Allogeneic primary mesenchymal stem/stromal cell aggregates within poly(N-isopropylacrylamide-co-acrylic acid) hydrogel for osteochondral regeneration[J]. APPLIED MATERIALS TODAY,2020,18:16.
APA Zhang, Jiabin.,Zhang, Ming.,Lin, Rongcai.,Yun, Seonho.,Du, Yuguang.,...&Zhang, Hu.(2020).Allogeneic primary mesenchymal stem/stromal cell aggregates within poly(N-isopropylacrylamide-co-acrylic acid) hydrogel for osteochondral regeneration.APPLIED MATERIALS TODAY,18,16.
MLA Zhang, Jiabin,et al."Allogeneic primary mesenchymal stem/stromal cell aggregates within poly(N-isopropylacrylamide-co-acrylic acid) hydrogel for osteochondral regeneration".APPLIED MATERIALS TODAY 18(2020):16.
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