CAS OpenIR
Charge exclusion as a strategy to control retention of small proteins in polyelectrolyte-modified ultrafiltration membranes
Ji, Mingbo1; Li, Xianhui1; Omidvarkordshouli, Maryam1; Sigurdardottir, Sigyn Bjork1; Woodley, John M.1; Daugaard, Anders Egede2; Luo, Jianquan3; Pinelo, Manuel1
2020-09-15
Source PublicationSEPARATION AND PURIFICATION TECHNOLOGY
ISSN1383-5866
Volume247Pages:9
AbstractWhile retention in commercial ultrafiltration membranes is commonly governed by size exclusion, addition of charged polyelectrolytes to the membrane surface has been proposed as a facile and inexpensive method to modulate retention during filtration of charged compounds, such as proteins. This study demonstrates that selected combinations of common polyelectrolytes can be efficiently used to control the retention of proteins. We show how the retention of positively charged lysozyme increased from similar to 7% to similar to 50% when a negatively charged polyelectrolyte was deposited on a commercial polysulfone membrane, most probably due to a combination of associated pore narrowing effect and protein adsorption onto the membrane. By contrast, the retention decreased again (from similar to 50% to similar to 30%) upon further addition of a positively charged polyelectrolyte onto the membrane. The electrostatic repulsion between the positively charged surface and proteins prevented protein adsorption onto the membrane, while pore size was still large enough that size exclusion was limited. The retention decreased further to similar to 22% when increasing amounts of polyelectrolytes of the same charge as the protein were deposited on the membrane. In addition, variations in pH revealed that the negative effect of polyelectrolytes of the same charge as the protein on lysozyme retention was reversed when the charge density of lysozyme reached a certain level beyond which the positively charged electrolyte promoted dramatic increases of rejection. The results of this study suggest that use of polyelectrolyte-modified ultrafiltration membranes with a much larger pore size than the charged solute to be filtered could be a strategy to control retention of such solutes by minimizing the effect of size exclusion and fouling.
KeywordLysozyme Polyelectrolyte deposition Electrostatic repulsion UF
DOI10.1016/j.seppur.2020.116936
Language英语
WOS KeywordHOLLOW-FIBER MEMBRANES ; NANOFILTRATION MEMBRANE ; SURFACE MODIFICATION ; TRANSPORT ; PERFORMANCE ; SEPARATION ; DOPAMINE
Funding ProjectChinese Scholarship Council[201704910984] ; Villum Foundation (Villum Experiment grant)[00028005]
WOS Research AreaEngineering
WOS SubjectEngineering, Chemical
Funding OrganizationChinese Scholarship Council ; Villum Foundation (Villum Experiment grant)
WOS IDWOS:000536142200006
PublisherELSEVIER
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/40833
Collection中国科学院过程工程研究所
Corresponding AuthorLuo, Jianquan; Pinelo, Manuel
Affiliation1.Tech Univ Denmark, Proc & Syst Engn Ctr PROSYS, Dept Chem & Biochem Engn, Bldg 229, DK-2800 Lyngby, Denmark
2.Tech Univ Denmark, Danish Polymer Ctr, Dept Chem & Biochem Engn, Bldg 229, DK-2800 Lyngby, Denmark
3.Chinese Acad Sci, Univ Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Ji, Mingbo,Li, Xianhui,Omidvarkordshouli, Maryam,et al. Charge exclusion as a strategy to control retention of small proteins in polyelectrolyte-modified ultrafiltration membranes[J]. SEPARATION AND PURIFICATION TECHNOLOGY,2020,247:9.
APA Ji, Mingbo.,Li, Xianhui.,Omidvarkordshouli, Maryam.,Sigurdardottir, Sigyn Bjork.,Woodley, John M..,...&Pinelo, Manuel.(2020).Charge exclusion as a strategy to control retention of small proteins in polyelectrolyte-modified ultrafiltration membranes.SEPARATION AND PURIFICATION TECHNOLOGY,247,9.
MLA Ji, Mingbo,et al."Charge exclusion as a strategy to control retention of small proteins in polyelectrolyte-modified ultrafiltration membranes".SEPARATION AND PURIFICATION TECHNOLOGY 247(2020):9.
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