CAS OpenIR
Genotyping of 30 kinds of cutaneous human papillomaviruses by a multiplex microfluidic loop-mediated isothermal amplification and visual detection method
Wang,Yining1,2,3,4,5; Ge,Ge1,2,3,4,5,6; Mao,Rui6; Wang,Zhuo6; Sun,Yu-Zhe7; Du,Yu-Guang6; Gao,Xing-Hua1,2,3,4,5; Qi,Rui-Qun1,2,3,4,5; Chen,Hong-Duo1,2,3,4,5
2020-07-09
Source PublicationVirology Journal
Volume17Issue:1
AbstractAbstractBackgroundHuman papillomaviruses (HPVs), a group of non-enveloped small viruses with double-stranded circular DNA which lead to multiple skin diseases such as benign warts, are commonly seen in clinics. The current HPV detection systems aim mainly at mucosal HPVs, however, an efficient clinical approach for cutaneous HPVs detection is lacking.ObjectivesTo establish a rapid detection system for cutaneous HPVs using a colorimetric loop-mediated isothermal amplification (LAMP) with hydroxynaphthol blue (HNB) dye in combination with microfluidic technology.MethodsL1 DNA sequences of the 30 cutaneous HPVs were chemically synthesized, and LAMP primers against L1 DNA were designed with use of an online LAMP designing tool. Isothermal amplification was performed with use of a water bath and the amplification results were inspected with the naked eye. Using PCR sequencing as a control method, the specificity and sensitivity of the new detection system were obtained by detecting clinical samples.ResultsThe lower detection limit of the LAMP assay was 107 viral DNA copies/μl when tested on synthesized L1 DNA sequences, which was better than the conventional PCR. Compared to PCR sequencing, the sensitivity of HPV27, HPV2, HPV1, HPV57, HPV3, HPV4, HPV7 and HPV75 genotypes detections were 100%, whereas the specificity was 34.55, 45.12, 95.83, 98.59 and 97.62% respectively, when tested on clinical samples.ConclusionsThe new cutaneous type HPV detection system is characterized by both a good sensitivity and specificity compared to conventional methods.
KeywordHPV Detection LAMP Microfluidic Genotyping
DOI10.1186/s12985-020-01373-3
Language英语
WOS IDBMC:10.1186/s12985-020-01373-3
PublisherBioMed Central
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Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/41029
Collection中国科学院过程工程研究所
Corresponding AuthorSun,Yu-Zhe; Du,Yu-Guang; Gao,Xing-Hua; Qi,Rui-Qun
Affiliation1.The First Hospital of China Medical University; Department of Dermatology
2.NHC Key Laboratory of Immunodermatology (China Medical University)
3.Key Laboratory of Immunodermatology (China Medical University), Ministry of Education
4.Key Laboratory of Immunodermatology
5.Key Laboratory of Immunodermatology (China Medical University), Department of education of Liaoning Province
6.Chinese Academy of Sciences; Key Laboratory of Biopharmaceutical Production & Formulation Engineering, PLA and State Key Laboratory of Biochemical Engineering, Institute of Process Engineering
7.Dermatology Hospital of Southern Medical University
Recommended Citation
GB/T 7714
Wang,Yining,Ge,Ge,Mao,Rui,et al. Genotyping of 30 kinds of cutaneous human papillomaviruses by a multiplex microfluidic loop-mediated isothermal amplification and visual detection method[J]. Virology Journal,2020,17(1).
APA Wang,Yining.,Ge,Ge.,Mao,Rui.,Wang,Zhuo.,Sun,Yu-Zhe.,...&Chen,Hong-Duo.(2020).Genotyping of 30 kinds of cutaneous human papillomaviruses by a multiplex microfluidic loop-mediated isothermal amplification and visual detection method.Virology Journal,17(1).
MLA Wang,Yining,et al."Genotyping of 30 kinds of cutaneous human papillomaviruses by a multiplex microfluidic loop-mediated isothermal amplification and visual detection method".Virology Journal 17.1(2020).
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