CAS OpenIR
Competitive annealing mediated isothermal amplification (CAMP) for rapid and simple detection of Listeria monocytogenes in milk
Li, Wei5; Mao, Rui2; Yue, Xiqing1; Wu, Junrui1; Wu, Rina1; Qiao, Yu3; Peng, Qing3; Shi, Bo3; Luo, Yong4; Chen, Xu1; Du, Yuguang2
2020-11-01
Source PublicationFOOD CONTROL
ISSN0956-7135
Volume117Pages:7
AbstractListeria monocytogenes (L. monocytogenes ) is widespread in nature and considered an important foodborne pathogen. And it can cause listeriosis, which can lead to miscarriage, neonatal death, septicemia and meningitis. Therefore, the appropriate detection methods are needed to effectively control and prevent this pathogenic bacterium. Competitive annealing mediated isothermal amplification (CAMP) is a novel nucleic acid-based detection technology that amplifies DNA with high sensitivity and specificity under isothermal conditions. The aim of this study was to develop a CAMP assay for rapid and simple detection of L. monocytogenes in milk. In this system, a pair of inner primers was designed to specifically target hlyA gene of L. monocytogenes. For further optimization, loop primers and outer primers were designed to accelerate CAMP reaction. Compared with conventional PCR method, the CAMP assay has the same specificity and higher sensitivity. All 8 strains of L. monocytogenes yielded positive results using the CAMP assay and showed no cross reaction with 26 tested non L. monocytogenes strains. The detection limit of the CAMP assay was 1.0 x 10(2) CFU/mL for pure bacterial, which was 10-fold more sensitive than PCR. Additionally, the CAMP method with the enrichment step could reliably detect L. monocytogenes in artificially contaminated milk samples at concentration as low as 1 CFU/mL. These results indicate the developed CAMP is a potential useful method for rapid detection of L. monocytogenes in milk samples.
KeywordCompetitive annealing mediated isothermal amplification L.monocytogenes Milk
DOI10.1016/j.foodcont.2020.107347
Language英语
WOS KeywordREAL-TIME PCR ; MULTIPLEX PCR ; FOOD ; SALMONELLA ; STORAGE
Funding ProjectNational key RD project[2018YFD0501403] ; Youth Project of the Education Department of Liaoning Province, China[LSNQN201911]
WOS Research AreaFood Science & Technology
WOS SubjectFood Science & Technology
Funding OrganizationNational key RD project ; Youth Project of the Education Department of Liaoning Province, China
WOS IDWOS:000541152600014
PublisherELSEVIER SCI LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/41177
Collection中国科学院过程工程研究所
Corresponding AuthorChen, Xu; Du, Yuguang
Affiliation1.Shenyang Agr Univ, Food Sci Coll, Shenyang 110866, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
3.Chinese Acad Agr Sci, Feed Res Inst, Beijing 100081, Peoples R China
4.Dalian Univ Technol, Sch Pharmaceut Sci & Technol, Dalian 116024, Peoples R China
5.Shenyang Agr Univ, Dept Publicat, Shenyang 110866, Peoples R China
Recommended Citation
GB/T 7714
Li, Wei,Mao, Rui,Yue, Xiqing,et al. Competitive annealing mediated isothermal amplification (CAMP) for rapid and simple detection of Listeria monocytogenes in milk[J]. FOOD CONTROL,2020,117:7.
APA Li, Wei.,Mao, Rui.,Yue, Xiqing.,Wu, Junrui.,Wu, Rina.,...&Du, Yuguang.(2020).Competitive annealing mediated isothermal amplification (CAMP) for rapid and simple detection of Listeria monocytogenes in milk.FOOD CONTROL,117,7.
MLA Li, Wei,et al."Competitive annealing mediated isothermal amplification (CAMP) for rapid and simple detection of Listeria monocytogenes in milk".FOOD CONTROL 117(2020):7.
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