CAS OpenIR
Three dimensional polyvinyl alcohol scaffolds modified with collagen for HepG2 cell culture
Meng, Di1; Lei, Xiongxin2; Li, Yang2,3; Kong, Yingjun2; Huang, Dawei1; Zhang, Guifeng2
2020-06-24
Source PublicationJOURNAL OF BIOMATERIALS APPLICATIONS
ISSN0885-3282
Pages12
AbstractThe creation of in vitro functional hepatic tissue simulating micro environmental niche of the native liver is a keen area of research due to its demand in bioartificial liver. However, it is still unclear how to maintain benign cell function while achieving the sufficient cell quantity. In this work, we aim to prepare a novel scaffold for the culture of HepG2 cells, a liver cell line, by modifying polyvinyl alcohol (PVA) scaffold with collagen (COL). PVA is a kind of synthetic biostable polymer with high hydrophilicity in the human body, has been widely used in the biomedical field. However, the use of PVA is limited in cell cultures due to lack of biologically active functional groups. In this study, amino silane (KH-550), glutaraldehyde and native type I collagen were used to modify three-dimensional PVA scaffold to establish a suitable composite scaffold for hepatocyte culture. Three types of composite scaffolds were prepared for different collagen content, named as PVA/COL (0.2%), PVA/COL (0.5%) and PVA/COL (0.8%), respectively. The composite scaffolds were characterized by SEM, XPS, FTIR, MS, porosity estimation and water contact angle measurement. The PVA/COL (0.8%) scaffolds had the highest collagen content of 12.13%. The composite scaffold showed high porosity with interconnected pores. Furthermore, the biocompatibility between HepG2 cells and scaffolds was evaluated by the ability of cell proliferation, albumin secretion, as well as urea synthesis. The coating of collagen on PVA scaffolds promoted hydrophilicity and HepG2 cell adhesion. Additionally, enhanced cell proliferation, increased albumin secretion and urea synthesis were observed in HepG2 cells growing on collagen-coated three-dimensional PVA scaffolds.
KeywordPolyvinyl alcohol scaffold collagen modification composite hepatocyte culture
DOI10.1177/0885328220933505
Language英语
WOS KeywordLIVER SUPPORT-SYSTEMS ; POTENTIAL HEPATOCYTE CARRIERS ; BIOARTIFICIAL LIVER ; I COLLAGEN ; FAILURE ; TRANSPLANTATION ; MODEL
Funding ProjectNational Key R&D program of China[2018YFA0108200] ; National Key R&D program of China[2018YF C1106400] ; National Key R&D program of China[2017YFF0207800] ; Science and Technology Program of Guangzhou[201803010086] ; Collaborative Innovation Projects of Marine Economic Innovation and Development Demonstration City of Haikou[HHCL201804]
WOS Research AreaEngineering ; Materials Science
WOS SubjectEngineering, Biomedical ; Materials Science, Biomaterials
Funding OrganizationNational Key R&D program of China ; Science and Technology Program of Guangzhou ; Collaborative Innovation Projects of Marine Economic Innovation and Development Demonstration City of Haikou
WOS IDWOS:000543095900001
PublisherSAGE PUBLICATIONS LTD
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Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/41276
Collection中国科学院过程工程研究所
Corresponding AuthorHuang, Dawei; Zhang, Guifeng
Affiliation1.Nankai Univ, Coll Life Sci, 94 Weijin Rd, Tianjin 300071, Peoples R China
2.Inst Proc Engn, Natl Key Lab Biochem Engn, 1 Bei Er Tiao, Beijing 100190, Peoples R China
3.Southern Med Univ, Zhujiang Hosp, Inst Regenerat Med, Guangzhou, Peoples R China
Recommended Citation
GB/T 7714
Meng, Di,Lei, Xiongxin,Li, Yang,et al. Three dimensional polyvinyl alcohol scaffolds modified with collagen for HepG2 cell culture[J]. JOURNAL OF BIOMATERIALS APPLICATIONS,2020:12.
APA Meng, Di,Lei, Xiongxin,Li, Yang,Kong, Yingjun,Huang, Dawei,&Zhang, Guifeng.(2020).Three dimensional polyvinyl alcohol scaffolds modified with collagen for HepG2 cell culture.JOURNAL OF BIOMATERIALS APPLICATIONS,12.
MLA Meng, Di,et al."Three dimensional polyvinyl alcohol scaffolds modified with collagen for HepG2 cell culture".JOURNAL OF BIOMATERIALS APPLICATIONS (2020):12.
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