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Novel keratinolytic enzymes, discovered from a talented and efficient bacterial keratin degrader
Huang, Yuhong1,4; Lezyk, Mateusz1,5; Herbst, Florian-Alexander2; Busk, Peter Kamp1,3; Lange, Lene1,6
2020-06-22
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume10Issue:1Pages:11
AbstractHuge quantities of keratinaceous waste are a substantial and almost totally unexploited protein resource which could be upgraded for use as high value-added products by efficient keratinolytic enzymes. In this study, we found that Bacillus sp. 8A6 can efficiently degrade chicken feather after 24h growth. According to phylogenetic analysis, the strain (formerly identified as Bacillus pumilus 8A6) belongs to the B. pumilus species clade but it is more closely related to B. safensis. Hotpep predicted 233 putative proteases from Bacillus sp. 8A6 genome. Proteomic analysis of culture broths from Bacillus sp. 8A6 cultured on chicken feathers or on a mixture of bristles and hooves showed high abundance of proteins with functions related to peptidase activity. Five proteases (one from family M12, one from family S01A, two from family S08A and one from family T3) and four oligopeptide and dipeptide binding proteins were highly expressed when Bacillus sp. 8A6 was grown in keratin media compared to LB medium. This study is the first to report that bacterial proteases in families M12, S01A and T3 are involved in keratin degradation together with proteases from family S08.
DOI10.1038/s41598-020-66792-2
Language英语
WOS KeywordM12 METALLOPROTEASE ; MICROBIAL GENOMES ; BACILLUS ; DEGRADATION ; DECOMPOSITION ; PURIFICATION ; HYDROLYSIS ; MYROILYSIN ; PREDICTION ; PROTEASES
Funding ProjectDanish Innovation Fund[1308-00015B]
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
Funding OrganizationDanish Innovation Fund
WOS IDWOS:000543966000012
PublisherNATURE PUBLISHING GROUP
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Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/41301
Collection中国科学院过程工程研究所
Corresponding AuthorLange, Lene
Affiliation1.Tech Univ Denmark, Dept Biotechnol & Biomed, Bldg 224, DK-2800 Lyngby, Denmark
2.Aalborg Univ, Ctr Microbial Communities, Dept Chem & Biosci, Fredrik Bajers Vej 7H, DK-9220 Aalborg, Denmark
3.Roskilde Univ, Dept Sci & Environm, Univ Vej 1, DK-4000 Roskilde, Denmark
4.Chinese Acad Sci, Inst Proc Engn, State Key Lab Multiphase Complex Syst, Beijing Key Lab Ion Liquids Clean Proc,Key Lab Gr, Beijing 100190, Peoples R China
5.Poznan Univ Tech, Fac Environm Engn & Energy, Water Supply & Bioecon Div, Berdychowo 4, PL-60965 Poznan, Poland
6.Bioecon Res & Advisory, Karensgade 5, DK-2500 Valby, Denmark
Recommended Citation
GB/T 7714
Huang, Yuhong,Lezyk, Mateusz,Herbst, Florian-Alexander,et al. Novel keratinolytic enzymes, discovered from a talented and efficient bacterial keratin degrader[J]. SCIENTIFIC REPORTS,2020,10(1):11.
APA Huang, Yuhong,Lezyk, Mateusz,Herbst, Florian-Alexander,Busk, Peter Kamp,&Lange, Lene.(2020).Novel keratinolytic enzymes, discovered from a talented and efficient bacterial keratin degrader.SCIENTIFIC REPORTS,10(1),11.
MLA Huang, Yuhong,et al."Novel keratinolytic enzymes, discovered from a talented and efficient bacterial keratin degrader".SCIENTIFIC REPORTS 10.1(2020):11.
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