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Efficient ex-situ biosynthesis of vitamin B-12 by Propionibacterium freudenreichii using membrane separation coupling technology
Wang, Ziqiang; Xu, Guoxia; Du, Wei; Zhang, Yuhan; Wang, Yunshan; Su, Zhiguo
2020-09-15
Source PublicationBIOCHEMICAL ENGINEERING JOURNAL
ISSN1369-703X
Volume161Pages:7
AbstractPropionibacterium freudenreichii has been successfully applied to industrial anaerobic production of vitamin B-12. In the traditional fed-batch fermentation process, 5,6-dimethylbenzimidazole (DMB) is used as a precursor. However, DMB can inhibit the synthesis of vitamin B-12 metabolic intermediates, which is not conducive to semicontinuous fermentation of P. freudenreichii. To improve the fermentation efficiency, vitamin B-12 ex-situ biosynthesis was proposed and a membrane separation coupling fermentation technology was designed with adenosylcobinamide (Ado-cbi) as the indicator signal. The results showed that P. freudenreichii cells should be partly separated online at 84 h with 0.22-mu m spiral membrane. The fourfold concentrated suspension was suitable for ex-situ biosynthesis of vitamin B-12 with the addition of 3.60 mg L-1 DMB at 30 degrees C and pH 7.0. In the semi-continuous coupling fermentation process, vitamin B-12 concentration reached 56.76 +/- 3.86 mg L-1 , an improvement of 161.6 %, while reutilizing fermentation wastewater and improving the anaerobic fermentation efficiency of P. freudenreichii.
KeywordPropionibacterium freudenreichii Vitamin B-12 Adenosylcobinamide Ex situ biosynthesis Membrane separation coupling fermentation
DOI10.1016/j.bej.2020.107688
Language英语
WOS KeywordPROPIONIC-ACID ; COBALAMIN VITAMIN-B-12 ; MICROBIAL-PRODUCTION ; CONTROL STRATEGY ; FERMENTATION ; REMOVAL ; ENZYME
Funding ProjectNational Natural Science Foundation of China[21506227]
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
Funding OrganizationNational Natural Science Foundation of China
WOS IDWOS:000552131300037
PublisherELSEVIER
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Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/41458
Collection中国科学院过程工程研究所
Corresponding AuthorWang, Yunshan
AffiliationChinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Wang, Ziqiang,Xu, Guoxia,Du, Wei,et al. Efficient ex-situ biosynthesis of vitamin B-12 by Propionibacterium freudenreichii using membrane separation coupling technology[J]. BIOCHEMICAL ENGINEERING JOURNAL,2020,161:7.
APA Wang, Ziqiang,Xu, Guoxia,Du, Wei,Zhang, Yuhan,Wang, Yunshan,&Su, Zhiguo.(2020).Efficient ex-situ biosynthesis of vitamin B-12 by Propionibacterium freudenreichii using membrane separation coupling technology.BIOCHEMICAL ENGINEERING JOURNAL,161,7.
MLA Wang, Ziqiang,et al."Efficient ex-situ biosynthesis of vitamin B-12 by Propionibacterium freudenreichii using membrane separation coupling technology".BIOCHEMICAL ENGINEERING JOURNAL 161(2020):7.
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