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| Potential of Using Cell-Free DNA and miRNA in Breast Milk to Screen Early Breast Cancer | |
| Song, Qinghe1,2; Zhang, Yingying3; Liu, Hongtao4; Du, Yuguang1,2 | |
| 2020-07-03 | |
| Source Publication | BIOMED RESEARCH INTERNATIONAL
 |
| ISSN | 2314-6133 |
| Volume | 2020Pages:11 |
| Abstract | Objective. An ideal sample source is critical for more reliable and sensitive early detection of nucleic acid changes associated with breast cancer. Breast milk (BM) is a good noninvasive origin for genetic testing of early breast cancer, but cells in BM are easily disintegrated. So we investigate here whether cell-free nucleic acid (cfNA) exists in BM in a more stable form and whether the quality of BM cfNA is good enough for genetic testing.Methods. A self-designed qRT-PCR method was used to measure the existence and abundance of cfDNA. Quality of cfDNA and cfRNA were detected by capillary electrophoresis. Whole genome bisulfite sequencing and miRNA sequencing were used to explore the sources of cfDNA and cell-free miRNA in BM. The copy number analysis andz-test based on whole genome sequencing data were used to determine the integrity of genetic information in BM cfNA.Results. We found that cell-free DNA and miRNA exist in the studied breast milk samples in a stable form that can tolerate incubation of BM at room temperature for at least 7 days. These cell-free nucleic acids come mainly from breast-derived cells and contain genetic information as good integrity as in BM cells. We further listed some candidate miRNAs as potential biomarkers for research of early breast cancer screening by analysis of previous reports and our data.Conclusions. Our results suggest that cfDNA and cell-free miRNA in BM might be new noninvasive sample sources for finding early alterations of nucleic acid associated with the initiation and progression of breast cancer. |
| DOI | 10.1155/2020/8126176 |
| Language | 英语 |
| WOS Keyword | LIQUID BIOPSIES ; MICRORNAS ; PLASMA ; EXPRESSION ; BIOMARKERS ; DEREGULATION ; METHYLATION ; ANEUPLOIDY ; DIAGNOSIS ; TISSUE |
| Funding Project | National Natural Science Foundation, China[31700705] ; National Natural Science Foundation, China[11902090] |
| WOS Research Area | Biotechnology & Applied Microbiology ; Research & Experimental Medicine |
| WOS Subject | Biotechnology & Applied Microbiology ; Medicine, Research & Experimental |
| Funding Organization | National Natural Science Foundation, China |
| WOS ID | WOS:000552800700001 |
| Publisher | HINDAWI LTD |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/41583 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Song, Qinghe; Du, Yuguang |
| Affiliation | 1.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, PLA, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Key Lab Biopharmaceut Prod & Formulat Engn, Inst Proc Engn, PLA, Beijing 100190, Peoples R China 3.Natl Res Ctr Rehabil Tech Aids, Beijing Key Lab Rehabil Tech Aids Old Age Disabil, Beijing 100176, Peoples R China 4.Hubei Univ Chinese Med, Wuhan 430065, Peoples R China |
| Recommended Citation GB/T 7714 | Song, Qinghe,Zhang, Yingying,Liu, Hongtao,et al. Potential of Using Cell-Free DNA and miRNA in Breast Milk to Screen Early Breast Cancer[J]. BIOMED RESEARCH INTERNATIONAL,2020,2020:11. |
| APA | Song, Qinghe,Zhang, Yingying,Liu, Hongtao,&Du, Yuguang.(2020).Potential of Using Cell-Free DNA and miRNA in Breast Milk to Screen Early Breast Cancer.BIOMED RESEARCH INTERNATIONAL,2020,11. |
| MLA | Song, Qinghe,et al."Potential of Using Cell-Free DNA and miRNA in Breast Milk to Screen Early Breast Cancer".BIOMED RESEARCH INTERNATIONAL 2020(2020):11. |
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