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A Novel Particulate Delivery System Based on Antigen-Zn2+ Coordination Interactions Enhances Stability and Cellular Immune Response of Inactivated Foot and Mouth Disease Virus Shuai
Li, Shuai1,2; Yang, Yanli1; Lin, Xuan1,2; Li, Zhengjun1; Ma, Guanghui1; Su, Zhiguo1; Zhang, Songping1
2020-08-03
Source PublicationMOLECULAR PHARMACEUTICS
ISSN1543-8384
Volume17Issue:8Pages:2952-2963
Abstract

The interactions between antigen and adjuvant were among the most significant factors influencing the immunogenicity of vaccines, especially for unstable antigens like inactivated foot and mouth disease virus (iFMDV). Here we propose a novel antigen delivery pattern based on the coordination interaction between transition metal ions Zn2+ chelated to chitosan nanoparticles and iFMDV, which is known to be rich in histidine. The zinc chelated chitosan particles (CP-PEI-Zn) were prepared by crosslinking chitosan particles (CP) with sodium tripolyphosphate (TPP), modifying with metal chelator polyethylenimine (PEI), and subsequent chelating of Zn2+. The coordination interaction was confirmed by analyzing the adsorption and desorption behavior of iFMDV on CP-PEI-Zn by high-performance size exclusion chromatography (HPSEC), while the CP-PEI without chelating Zn2+ loads iFMDV mainly through electrostatic interactions. The iFMDV loaded on CP-PEI-Zn showed better thermal stability than that on CP-PEI, as revealed by a slightly higher transition temperature (T-m) related to iFMDV dissociation. After subcutaneous immunization in female Balb/C mice, antigens loaded on CP-PEI and CP-PEI-Zn all induced higher specific antibody titers, better activation of B lymphocytes, and more effector-memory T cells proliferation than the free antigen and iFMDV adjuvanted with ISA 206 emulsion did. Moreover, CP-PEI-Zn showed superior efficacy to CP-PEI in promoting the proliferation of effector-memory T cells and secretion of cytokines, indicating a more potent cellular immune response. In summary, the CP-PEI-Zn stabilized the iFMDV after loading and promoted both humoral and cellular immune responses, thus reflecting its potential to be a promising adjuvant for the iFMDV vaccine and other unstable viral antigens.

KeywordChitosan Particles Coordination Binding Foot And Mouth Disease Virus Thermal Stability Cellular Immunity
DOI10.1021/acs.molpharmaceut.0c00365
Language英语
WOS KeywordVaccine Delivery ; Size ; Adsorption ; Adjuvants ; Microspheres ; Dissociation ; Recognition ; Mechanism ; Provides ; Binding
Funding ProjectNational Natural Sciences Foundation of China[21821005] ; National Natural Sciences Foundation of China[31970872] ; National Natural Sciences Foundation of China[21808226]
WOS Research AreaResearch & Experimental Medicine ; Pharmacology & Pharmacy
WOS SubjectMedicine, Research & Experimental ; Pharmacology & Pharmacy
Funding OrganizationNational Natural Sciences Foundation of China
WOS IDWOS:000558726700017
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/41655
Collection中国科学院过程工程研究所
Corresponding AuthorSu, Zhiguo; Zhang, Songping
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Li, Shuai,Yang, Yanli,Lin, Xuan,et al. A Novel Particulate Delivery System Based on Antigen-Zn2+ Coordination Interactions Enhances Stability and Cellular Immune Response of Inactivated Foot and Mouth Disease Virus Shuai[J]. MOLECULAR PHARMACEUTICS,2020,17(8):2952-2963.
APA Li, Shuai.,Yang, Yanli.,Lin, Xuan.,Li, Zhengjun.,Ma, Guanghui.,...&Zhang, Songping.(2020).A Novel Particulate Delivery System Based on Antigen-Zn2+ Coordination Interactions Enhances Stability and Cellular Immune Response of Inactivated Foot and Mouth Disease Virus Shuai.MOLECULAR PHARMACEUTICS,17(8),2952-2963.
MLA Li, Shuai,et al."A Novel Particulate Delivery System Based on Antigen-Zn2+ Coordination Interactions Enhances Stability and Cellular Immune Response of Inactivated Foot and Mouth Disease Virus Shuai".MOLECULAR PHARMACEUTICS 17.8(2020):2952-2963.
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