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A novel rapid modularized hepatitis B core virus-like particle-based platform for personalized cancer vaccine preparation via fixed-point coupling
Ji, Mei1,2; Zhu, Jie1,3,4,5; Xie, Xi-xiu1; Liu, Dong-qun1,2; Wang, Bin1; Yu, Zhuo6; Liu, Rui-tian1
2020-08-01
Source PublicationNANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
ISSN1549-9634
Volume28Pages:13
Abstract

Personalized cancer vaccine which targets neoepitopes shows great promise for cancer treatment. However, rapid preparation is a critical challenge for clinical application of personalized cancer vaccine. Genetic recombination and chemical modification are a time-consuming "trial and error" pattern for making vaccines. Here we first constructed a platform for peptide vaccine preparation by inserting SpyCatcher into the major immunodominant region (MIR) of hepatitis B core protein (HBc) (1-183). The resulted recombinant protein HBc((1-183))SpyCatcher (HBc((1-183))-S) assembled to virus-like particles (VLPs) and readily bound to SpyTag conjugated with OVA epitope peptides by just mixing, forming HBc((1-183))-S-OVA. HBc((1-183))-S-OVA VLPs effectively induced dendritic cell maturation. Our further results indicated that HBc((1-183))-S-OVA VLPs vaccination inhibited tumor growth in both prophylactic and treatment ways in E.G7-OVA tumor bearing mice by generating significant OVA-specific cytotoxic T lymphocyte responses. Our study provides a simple, rapid, efficient and universal HBc-based platform for the preparation of personalized cancer vaccine. (C) 2020 Elsevier Inc. All rights reserved.

KeywordHepatitis b Core Protein Virus-like Particle (Vlp) Personalized Cancer Vaccine Spycatcher/spytag t Cell Epitope
DOI10.1016/j.nano.2020.102223
Language英语
WOS KeywordCross-presentation ; Peptide ; Delivery ; Antigen ; Immunogenicity ; Trafficking ; Generation ; Epitopes ; Immunity ; Size
Funding ProjectNational Natural Science Foundation of China[81371208] ; National Natural Science Foundation of China[81971073] ; National Natural Science Foundation of China[81903531] ; Chinese Academy of Sciences Strategic Pilot Project[XDA12040215]
WOS Research AreaScience & Technology - Other Topics ; Research & Experimental Medicine
WOS SubjectNanoscience & Nanotechnology ; Medicine, Research & Experimental
Funding OrganizationNational Natural Science Foundation of China ; Chinese Academy of Sciences Strategic Pilot Project
WOS IDWOS:000563777100001
PublisherELSEVIER
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/41829
Collection中国科学院过程工程研究所
Corresponding AuthorYu, Zhuo; Liu, Rui-tian
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China
2.Univ Chinese Acad Sci, Sch Chem Engn, Beijing, Peoples R China
3.Chinese Acad Med Sci, Inst Mat Med, State Key Lab Bioact Subst & Funct Nat Med, Beijing, Peoples R China
4.Chinese Acad Med Sci, Neurosci Ctr, Beijing, Peoples R China
5.Peking Union Med Coll, Beijing, Peoples R China
6.Beijing Tsinghua Changgung Hosp, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Ji, Mei,Zhu, Jie,Xie, Xi-xiu,et al. A novel rapid modularized hepatitis B core virus-like particle-based platform for personalized cancer vaccine preparation via fixed-point coupling[J]. NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE,2020,28:13.
APA Ji, Mei.,Zhu, Jie.,Xie, Xi-xiu.,Liu, Dong-qun.,Wang, Bin.,...&Liu, Rui-tian.(2020).A novel rapid modularized hepatitis B core virus-like particle-based platform for personalized cancer vaccine preparation via fixed-point coupling.NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE,28,13.
MLA Ji, Mei,et al."A novel rapid modularized hepatitis B core virus-like particle-based platform for personalized cancer vaccine preparation via fixed-point coupling".NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE 28(2020):13.
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