CAS OpenIR
Biosynthesis of Self-Assembled Proteinaceous Nanoparticles for Vaccination
Pan, Chao1; Wu, Jun1; Qing, Shuang2,3; Zhang, Xiao2; Zhang, Lulu1; Yue, Hua2; Zeng, Ming4; Wang, Bin4; Yuan, Zheng5; Qiu, Yefeng5; Ye, Huahu5; Wang, Dongshu1; Liu, Xiankai1; Sun, Peng1; Liu, Bo1; Feng, Erling1; Gao, Xiaoyong2; Zhu, Li1; Wei, Wei2,3; Ma, Guanghui2,3; Wang, Hengliang1
2020-09-02
Source PublicationADVANCED MATERIALS
ISSN0935-9648
Pages13
AbstractRecent years have seen enormous advances in nanovaccines for both prophylactic and therapeutic applications, but most of these technologies employ chemical or hybrid semi-biosynthetic production methods. Thus, production of nanovaccines has to date failed to exploit biology-only processes like complex sequential post-translational biochemical modifications and scalability, limiting the realization of the initial promise for offering major performance advantages and improved therapeutic outcomes over conventional vaccines. A Nano-B5 platform for in vivo production of fully protein-based, self-assembling, stable nanovaccines bearing diverse antigens including peptides and polysaccharides is presented here. Combined with the self-assembly capacities of pentamer domains from the bacterial AB(5)toxin and unnatural trimer peptides, diverse nanovaccine structures can be produced in commonEscherichia colistrains and in attenuated pathogenic strains. Notably, the chassis of these nanovaccines functions as an immunostimulant. After showing excellent lymph node targeting and immunoresponse elicitation and safety performance in both mouse and monkey models, the strong prophylactic effects of these nanovaccines against infection, as well as their efficient therapeutic effects against tumors are further demonstrated. Thus, the Nano-B5 platform can efficiently combine diverse modular components and antigen cargos to efficiently generate a potentially very large diversity of nanovaccine structures using many bacterial species.
KeywordAB(5)toxins biosynthesis conjugate vaccines nanovaccines self-assembled proteins
DOI10.1002/adma.202002940
Language英语
WOS KeywordVIRUS-LIKE PARTICLES ; CHOLERA-TOXIN ; O-ANTIGEN ; DELIVERY ; VACCINES ; NANOTECHNOLOGY ; ACTIVATION ; RECEPTOR ; DESIGN ; CELLS
Funding ProjectNational Natural Science Foundation of China[81930122] ; National Natural Science Foundation of China[81871314] ; National Natural Science Foundation of China[31700802] ; National Natural Science Foundation of China[21622608] ; National Natural Science Foundation of China[81773619] ; National Natural Science Foundation of China[21821005] ; National Science and Technology Major Project[2018ZX10101003-005] ; National Science and Technology Major Project[2018ZX09711003-013] ; National Key R&D Program of China[2017YFA0207900] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB29040303]
WOS Research AreaChemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS SubjectChemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter
Funding OrganizationNational Natural Science Foundation of China ; National Science and Technology Major Project ; National Key R&D Program of China ; Strategic Priority Research Program of the Chinese Academy of Sciences
WOS IDWOS:000565425700001
PublisherWILEY-V C H VERLAG GMBH
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/41876
Collection中国科学院过程工程研究所
Corresponding AuthorZhu, Li; Wei, Wei; Ma, Guanghui; Wang, Hengliang
Affiliation1.Beijing Inst Biotechnol, State Key Lab Pathogen & Biosecur, 20 Dongda St, Beijing 100071, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, 1 Bei Er Tiao, Beijing 100190, Peoples R China
3.Univ Chinese Acad Sci, Sch Chem Engn, 19A Yuquan Rd, Beijing 100049, Peoples R China
4.Natl Inst Food & Drug Control, 31 Huatuo Rd, Beijing 102629, Peoples R China
5.Acad Mil Med Sci, Anim Ctr, 20 Dongda St, Beijing 100071, Peoples R China
Recommended Citation
GB/T 7714
Pan, Chao,Wu, Jun,Qing, Shuang,et al. Biosynthesis of Self-Assembled Proteinaceous Nanoparticles for Vaccination[J]. ADVANCED MATERIALS,2020:13.
APA Pan, Chao.,Wu, Jun.,Qing, Shuang.,Zhang, Xiao.,Zhang, Lulu.,...&Wang, Hengliang.(2020).Biosynthesis of Self-Assembled Proteinaceous Nanoparticles for Vaccination.ADVANCED MATERIALS,13.
MLA Pan, Chao,et al."Biosynthesis of Self-Assembled Proteinaceous Nanoparticles for Vaccination".ADVANCED MATERIALS (2020):13.
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