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| Toxicological evaluation of ionic liquid in a biological functional tissue construct model based on nano-hydroxyapatite/chitosan/gelatin hybrid scaffolds | |
| Zhu, Jingjing1; Kong, Qian1; Zheng, Shuangshuang2; Wang, Yiwei3; Jiao, Zeren4; Nie, Yi2,5; Liu, Tianqing1; Song, Kedong1 | |
| 2020-09-01 | |
| Source Publication | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| ISSN | 0141-8130 |
| Volume | 158Pages:800-810 |
| Abstract | The application of ionic liquid is attracting more attentions as green replacement for volatile organic solvents. However, the toxic effects and risks of ionic liquid in different biological systems for human health and environ-ment are poorly evaluated. Among all ionic liquids, 1-ethyl-3-methylimidazolium diethylphosphate ([Emim] DEP-type) ionic liquid is still at the early phase of development, and its toxicity remains unclear. In this study, we fabricated a 3D biological functional tissue construct model based on nano-hydroxyapatite, chitosan and gel-atin hybrid scaffold and evaluated its toxic effects of [Emim]DEP-type ionic liquid. As a control group, the exam-ination of ionic liquid's toxic effects on the pre-osteoblast cell line (MC3T3-E1) was detected in 2D cultures. The MTT assay showed that [Emim]DEP-type ionic liquid inhibited the proliferation of cells on both 2D cultures and 3D tissue constructs. This effect was correlated with culturing time and concentration, while the IC50 on 3D scaf-folds (12,566, 9015, 7896 mu g/mL, at 24 h, 48 h and 72 h, respectively) was found significantly higher compared to 2D cultures (3959, 2226,1884 mu g/mL). Flow cytometry analysis and scanning electron microscope demonstrated that when [Emim]DEP-type ionic liquid acted on MC3T3-E1 cells for 48 h, the shape of 2D cells shrank, together with decreased surface adhesion. (C) 2020 Published by Elsevier B.V. |
| Keyword | Ionic liquid Cytotoxicity Three-dimensional tissue constructs |
| DOI | 10.1016/j.ijbiomac.2020.04.267 |
| Language | 英语 |
| WOS Keyword | FISH CELL-LINES ; 1-OCTYL-3-METHYLIMIDAZOLIUM BROMIDE ; MICROWAVE SYNTHESIS ; CYTOTOXICITY ; TOXICITY ; ACID ; ESTERIFICATION ; ECOTOXICOLOGY ; EXTRACTION ; CHLORIDE |
| Funding Project | National Natural Science Foundation of China[31670978/21676041] ; Fok Ying Tung Education Foundation[132027] ; State Key Laboratory of Fine Chemicals[KF1111] ; Natural Science Foundation of Liaoning[20180510028] ; Fundamental Research Funds for the Central Universities[DUT20ZD206] |
| WOS Research Area | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| WOS Subject | Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science |
| Funding Organization | National Natural Science Foundation of China ; Fok Ying Tung Education Foundation ; State Key Laboratory of Fine Chemicals ; Natural Science Foundation of Liaoning ; Fundamental Research Funds for the Central Universities |
| WOS ID | WOS:000564484800007 |
| Publisher | ELSEVIER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/41957 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Nie, Yi; Liu, Tianqing; Song, Kedong |
| Affiliation | 1.Dalian Univ Technol, Dalian R&D Ctr Stem Cell & Tissue Engn, State Key Lab Fine Chem, Dalian 116024, Peoples R China 2.Zhengzhou Inst Emerging Ind Technol, Zhengzhou 450000, Peoples R China 3.Univ Sydney, ANZAC Res Inst, Burns Res Grp, Concord, NSW 2139, Australia 4.Texas A&M Univ, Artie McFerrin Dept Chem Engn, College Stn, TX 77843 USA 5.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China |
| Recommended Citation GB/T 7714 | Zhu, Jingjing,Kong, Qian,Zheng, Shuangshuang,et al. Toxicological evaluation of ionic liquid in a biological functional tissue construct model based on nano-hydroxyapatite/chitosan/gelatin hybrid scaffolds[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2020,158:800-810. |
| APA | Zhu, Jingjing.,Kong, Qian.,Zheng, Shuangshuang.,Wang, Yiwei.,Jiao, Zeren.,...&Song, Kedong.(2020).Toxicological evaluation of ionic liquid in a biological functional tissue construct model based on nano-hydroxyapatite/chitosan/gelatin hybrid scaffolds.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,158,800-810. |
| MLA | Zhu, Jingjing,et al."Toxicological evaluation of ionic liquid in a biological functional tissue construct model based on nano-hydroxyapatite/chitosan/gelatin hybrid scaffolds".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 158(2020):800-810. |
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