CAS OpenIR
Toxicological evaluation of ionic liquid in a biological functional tissue construct model based on nano-hydroxyapatite/chitosan/gelatin hybrid scaffolds
Zhu, Jingjing1; Kong, Qian1; Zheng, Shuangshuang2; Wang, Yiwei3; Jiao, Zeren4; Nie, Yi2,5; Liu, Tianqing1; Song, Kedong1
2020-09-01
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
Volume158Pages:800-810
AbstractThe application of ionic liquid is attracting more attentions as green replacement for volatile organic solvents. However, the toxic effects and risks of ionic liquid in different biological systems for human health and environ-ment are poorly evaluated. Among all ionic liquids, 1-ethyl-3-methylimidazolium diethylphosphate ([Emim] DEP-type) ionic liquid is still at the early phase of development, and its toxicity remains unclear. In this study, we fabricated a 3D biological functional tissue construct model based on nano-hydroxyapatite, chitosan and gel-atin hybrid scaffold and evaluated its toxic effects of [Emim]DEP-type ionic liquid. As a control group, the exam-ination of ionic liquid's toxic effects on the pre-osteoblast cell line (MC3T3-E1) was detected in 2D cultures. The MTT assay showed that [Emim]DEP-type ionic liquid inhibited the proliferation of cells on both 2D cultures and 3D tissue constructs. This effect was correlated with culturing time and concentration, while the IC50 on 3D scaf-folds (12,566, 9015, 7896 mu g/mL, at 24 h, 48 h and 72 h, respectively) was found significantly higher compared to 2D cultures (3959, 2226,1884 mu g/mL). Flow cytometry analysis and scanning electron microscope demonstrated that when [Emim]DEP-type ionic liquid acted on MC3T3-E1 cells for 48 h, the shape of 2D cells shrank, together with decreased surface adhesion. (C) 2020 Published by Elsevier B.V.
KeywordIonic liquid Cytotoxicity Three-dimensional tissue constructs
DOI10.1016/j.ijbiomac.2020.04.267
Language英语
WOS KeywordFISH CELL-LINES ; 1-OCTYL-3-METHYLIMIDAZOLIUM BROMIDE ; MICROWAVE SYNTHESIS ; CYTOTOXICITY ; TOXICITY ; ACID ; ESTERIFICATION ; ECOTOXICOLOGY ; EXTRACTION ; CHLORIDE
Funding ProjectNational Natural Science Foundation of China[31670978/21676041] ; Fok Ying Tung Education Foundation[132027] ; State Key Laboratory of Fine Chemicals[KF1111] ; Natural Science Foundation of Liaoning[20180510028] ; Fundamental Research Funds for the Central Universities[DUT20ZD206]
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
Funding OrganizationNational Natural Science Foundation of China ; Fok Ying Tung Education Foundation ; State Key Laboratory of Fine Chemicals ; Natural Science Foundation of Liaoning ; Fundamental Research Funds for the Central Universities
WOS IDWOS:000564484800007
PublisherELSEVIER
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/41957
Collection中国科学院过程工程研究所
Corresponding AuthorNie, Yi; Liu, Tianqing; Song, Kedong
Affiliation1.Dalian Univ Technol, Dalian R&D Ctr Stem Cell & Tissue Engn, State Key Lab Fine Chem, Dalian 116024, Peoples R China
2.Zhengzhou Inst Emerging Ind Technol, Zhengzhou 450000, Peoples R China
3.Univ Sydney, ANZAC Res Inst, Burns Res Grp, Concord, NSW 2139, Australia
4.Texas A&M Univ, Artie McFerrin Dept Chem Engn, College Stn, TX 77843 USA
5.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Jingjing,Kong, Qian,Zheng, Shuangshuang,et al. Toxicological evaluation of ionic liquid in a biological functional tissue construct model based on nano-hydroxyapatite/chitosan/gelatin hybrid scaffolds[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2020,158:800-810.
APA Zhu, Jingjing.,Kong, Qian.,Zheng, Shuangshuang.,Wang, Yiwei.,Jiao, Zeren.,...&Song, Kedong.(2020).Toxicological evaluation of ionic liquid in a biological functional tissue construct model based on nano-hydroxyapatite/chitosan/gelatin hybrid scaffolds.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,158,800-810.
MLA Zhu, Jingjing,et al."Toxicological evaluation of ionic liquid in a biological functional tissue construct model based on nano-hydroxyapatite/chitosan/gelatin hybrid scaffolds".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 158(2020):800-810.
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