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A sustainable pH shift control strategy for efficient production of beta-poly(L-malic acid) with CaCO3 addition by Aureobasidium pullulans ipe-1
Cao, Weifeng1,2; Cao, Weilei1,2; Shen, Fei1,2; Luo, Jianquan1,2; Yin, Junxiang3; Qiao, Changsheng4; Wan, Yinhua1,2
2020-10-01
Source PublicationAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN0175-7598
Volume104Issue:20Pages:8691-8703
Abstractbeta-poly(L-malic acid) (PMLA) has attracted industrial interest for its potential applications in medicine and other industries. For a sustainable PMLA production, it requires replacing/reducing the CaCO3 usage, since the residual CaCO3 impeded the cells' utilization, and a large amount of commercially useless gypsum was accumulated. In this study, it was found that more glucose was converted into CO(2)using soluble alkalis compared with CaCO3 usage. Moreover, since the high ion strength and respiration effect of soluble alkalis also inhibited PMLA production, they could not effectively replace CaCO3. Furthermore, comparing the fermentations with different neutralizers (soluble alkali vs. CaCO3), it was found that the differential genes are mainly involved in the pathway of starch and sucrose metabolism, pentose and glucuronate interconversions, histidine metabolism, ascorbate and aldarate metabolism, and phagosome. In detail, in the case with CaCO3, 562 genes were downregulated and 262 genes were upregulated, and especially, those genes involved in energy production and conversion were downregulated by 26.7%. Therefore, the irreplaceability of CaCO3 was caused by its effect on the PMLA metabolic pathway rather than its usage as neutralizer. Finally, a combined pH shift control strategy with CaCO3 addition was developed. After the fermentation, 64.8 g/L PMLA and 38.9 g/L biomass were obtained with undetectable CaCO3 and less CO2 emission.
Keywordbeta-poly(L-malic acid) Aureobasidium pullulans Neutralizer Metabolic pathway
DOI10.1007/s00253-020-10815-5
Language英语
WOS KeywordEXPANDED-BED ADSORPTION ; GAMMA-AMINOBUTYRIC-ACID ; ION-EXCHANGE-RESIN ; MALIC-ACID ; POLY(BETA-MALIC ACID) ; POLYMALIC ACID ; SUCCINIC ACID ; FERMENTATION ; SEPARATION ; BROTH
Funding ProjectBeijing Natural Science Foundation, China[5182025] ; National Natural Science Foundation of China, China[21406240] ; National High Technology Research and Development Program of China[2015AA021002] ; National High Technology Research and Development Program of China[2014AA021005]
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
Funding OrganizationBeijing Natural Science Foundation, China ; National Natural Science Foundation of China, China ; National High Technology Research and Development Program of China
WOS IDWOS:000567768900004
PublisherSPRINGER
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/42007
Collection中国科学院过程工程研究所
Corresponding AuthorCao, Weifeng; Wan, Yinhua
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Sch Chem Engn, Beijing 100049, Peoples R China
3.China Natl Ctr Biotechnol Dev, Beijing 100036, Peoples R China
4.Tianjin Univ Sci & Technol, Coll Bioengn, Tianjin 300457, Peoples R China
Recommended Citation
GB/T 7714
Cao, Weifeng,Cao, Weilei,Shen, Fei,et al. A sustainable pH shift control strategy for efficient production of beta-poly(L-malic acid) with CaCO3 addition by Aureobasidium pullulans ipe-1[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2020,104(20):8691-8703.
APA Cao, Weifeng.,Cao, Weilei.,Shen, Fei.,Luo, Jianquan.,Yin, Junxiang.,...&Wan, Yinhua.(2020).A sustainable pH shift control strategy for efficient production of beta-poly(L-malic acid) with CaCO3 addition by Aureobasidium pullulans ipe-1.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,104(20),8691-8703.
MLA Cao, Weifeng,et al."A sustainable pH shift control strategy for efficient production of beta-poly(L-malic acid) with CaCO3 addition by Aureobasidium pullulans ipe-1".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 104.20(2020):8691-8703.
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