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Screening and structure study of active components of Astragalus polysaccharide for injection based on different molecular weights
Li, Ke1,2,4; Li, Shuying1,2,3; Du, Yuguang4; Qin, Xuemei1,2
2020-09-01
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN1570-0232
Volume1152Pages:13
AbstractAs a special traditional Chinese medicine, Astragalus polysaccharides for injection (APS, batch no. Zhunzi Z20040086) includes complex polysaccharide macromolecules that may increase the risk upon application. Although fingerprints for quality control are available, the specific active ingredients are unclear. Identifying the active components is the key to reduce the risk of adverse reactions of the drug. In this work, APS was mainly separated into two components, namely, macromolecular component (APS-I) and small molecular components (APS-II). The molecular weight measurement revealed that the average molecular weight of APS-I exceeded 500 kDa, and that of APS-II was 10 kDa. Monosaccharide-composition analysis revealed that APS-I consisted of glucose, galactose, arabinose, rhamnose, and galacturonic acid, with a ratio of approximately 1.5:1:5.4:0.08:0.1. Meanwhile, APS-II consisted of glucose, galactose, arabinose, rhamnose, and galacturonic acid, with a molar ratio of 9:1:1.4:0.04:0.001. Methylation, FT-IR, and NMR analysis indicated that the APS-I monosaccharide residue was linked as follows: D-Glcp-(1 ->, -> 4)-D-Glcp-(1 ->, -> 2)-L-Rhap-(1 ->, D-Araf-(1 ->, -> 5)-D-Araf-(1 ->, -> 2,5)-D-Araf-(1 ->, -> 4)-D-Galp-(1 -> . Meanwhile, the APS-II monosaccharide residue was connected as follows: alpha-D-Glcp-(1 ->,-> 4)-alpha-D-Glcp-(1 ->, -> 6)-alpha-D-Glcp-(1 ->, -> 4,6)-alpha-D-Glcp-(1 ->, -> 3,4,6)-alpha-D-Glcp-(1 ->, -> 2)-alpha-L-Rhap-(1 ->, alpha-D-Araf-(1 ->, -> 5)-alpha-D-Araf-(1 ->, -> 4)-beta-D-Galp-(1 -> . Screening experiments on their in vitro immunological activity showed that APS-II had stronger effect on innate and adaptive immunities than APS-I. In vivo animal experiments showed that APS-II can increase the leukocyte level of cyclophosphamide immunosuppressed mice and improve their immunomodulatory ability. Therefore, APS-II is the main active ingredient of APS and is expected to become a new generation of APS products.
KeywordAstragalus polysaccharides for injection Innate and adaptive immunity Proliferation Phagocytic Structural analysis
DOI10.1016/j.jchromb.2020.122255
Language英语
WOS KeywordIMMUNOMODULATORY ACTIVITY ; ANTIOXIDANT ACTIVITIES
Funding ProjectNational Natural Science Foundation of China[81872962] ; National Key R&D Program of China[2019YFC1710800] ; China Postdoctoral Science Foundation Project[2019M650851] ; Science and Technology Research Project of Shanxi Province (China)[2014ZD0401] ; Key Projects of Key Research and Development Plan in Shanxi (China)[201603D311101] ; Shanxi Province Technology Innovation Project of Excellent Talent (China)[201605D211030] ; Shanxi Province Technology Innovation Project of Excellent Talent (China)[201705D211020]
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
Funding OrganizationNational Natural Science Foundation of China ; National Key R&D Program of China ; China Postdoctoral Science Foundation Project ; Science and Technology Research Project of Shanxi Province (China) ; Key Projects of Key Research and Development Plan in Shanxi (China) ; Shanxi Province Technology Innovation Project of Excellent Talent (China)
WOS IDWOS:000566904300023
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/42026
Collection中国科学院过程工程研究所
Corresponding AuthorDu, Yuguang; Qin, Xuemei
Affiliation1.Shanxi Univ, Modern Res Ctr Tradit Chinese Med, Taiyuan 030006, Peoples R China
2.Shanxi Univ, Engn Minist, Key Lab Chem Biol & Mol, Taiyuan 030006, Peoples R China
3.Shanxi Univ, Coll Chem & Chem Engn, Taiyuan 030006, Peoples R China
4.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Li, Ke,Li, Shuying,Du, Yuguang,et al. Screening and structure study of active components of Astragalus polysaccharide for injection based on different molecular weights[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2020,1152:13.
APA Li, Ke,Li, Shuying,Du, Yuguang,&Qin, Xuemei.(2020).Screening and structure study of active components of Astragalus polysaccharide for injection based on different molecular weights.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1152,13.
MLA Li, Ke,et al."Screening and structure study of active components of Astragalus polysaccharide for injection based on different molecular weights".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1152(2020):13.
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