CAS OpenIR
Green preparation of hydrogel particles-in-emulsions for simultaneous enhancement of humoral and cell-mediated immunity
Zou, Yongjuan1,2; Li, Shuai1,2; Ngai, To3; Zhang, Songping1,2; Ma, Guanghui1,2,4,5; Wu, Jie1,2,4,5
2020-09-21
Source PublicationENGINEERING IN LIFE SCIENCES
ISSN1618-0240
Pages11
AbstractEmulsions are one of the most often used vaccine adjuvant formulations. Although they promote high humoral immunity, the induced cellular immunity is often poor, which restrict their application. To enhance the cellular immunity, some researchers have prepared mixed formulations by adding particles into the aqueous phase of emulsions. However, the particle preparation process usually involves the addition and removal of organic reagents, which is environmentally unfriendly and cumbersome. Moreover, the obtained vaccine adjuvant only induces limited cell-mediated immunity and humoral immunity compared with emulsion-adjuvanted vaccines. Herein, we developed a green and simple method for fabricating a novel nanoparticles-in-emulsions (NPE) formulation. Firstly, a temperature-sensitive hydrogel was used to prepare particles by self-solidification without additional crosslinking reagents. Secondly, the white oil was used as organic phase to avoid the particle washing procedures and organic solvent residues. Moreover, the effect of NPE as vaccine adjuvant was evaluated by using two veterinary vaccines as model antigens. NPE showed advantages than the conventional vaccine formulations in inducing both humoral and cellular immunity. This work provides a facile and broadly applicable approach for preparing nanoparticles-in-emulsions formulation, and presents an effective adjuvant for enhancing immunity against infectious diseases.
Keywordcellular immune responses green preparation humoral immunity oil-in-water emulsions quaternized chitosan particles
DOI10.1002/elsc.202000011
Language英语
WOS KeywordMOUTH-DISEASE VIRUS ; MEMBRANE EMULSIFICATION ; CHITOSAN MICROSPHERES ; ADJUVANT COMBINATION ; FMDV VACCINE ; RESPONSES ; DELIVERY ; MICROPARTICLES ; VETERINARY ; RELEASE
Funding ProjectNational Key R&D Program of China[2018YFC0311303] ; National Natural Science Foundation of China[21576268] ; National Natural Science Foundation of China[21776287] ; National Natural Science Foundation of China[21821005] ; Key Research Program of the Chinese Academy of Sciences[KFZD-SW-218] ; International Cooperation Project of the Chinese Academy of Sciences[122111KYSB20180021]
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
Funding OrganizationNational Key R&D Program of China ; National Natural Science Foundation of China ; Key Research Program of the Chinese Academy of Sciences ; International Cooperation Project of the Chinese Academy of Sciences
WOS IDWOS:000571690000001
PublisherWILEY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/42213
Collection中国科学院过程工程研究所
Corresponding AuthorWu, Jie
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, North 2nd St, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Sch Chem Engn, Beijing, Peoples R China
3.Chinese Univ Hong Kong, Dept Chem, Shatin, Hong Kong, Peoples R China
4.Chinese Acad Sci, Inst Proc Engn, PLA Key Lab Biopharmaceut Prod & Formulat Engn, Beijing, Peoples R China
5.Jiangsu Natl Synerget Innovat Ctr Adv Mat, Nanjing, Peoples R China
Recommended Citation
GB/T 7714
Zou, Yongjuan,Li, Shuai,Ngai, To,et al. Green preparation of hydrogel particles-in-emulsions for simultaneous enhancement of humoral and cell-mediated immunity[J]. ENGINEERING IN LIFE SCIENCES,2020:11.
APA Zou, Yongjuan,Li, Shuai,Ngai, To,Zhang, Songping,Ma, Guanghui,&Wu, Jie.(2020).Green preparation of hydrogel particles-in-emulsions for simultaneous enhancement of humoral and cell-mediated immunity.ENGINEERING IN LIFE SCIENCES,11.
MLA Zou, Yongjuan,et al."Green preparation of hydrogel particles-in-emulsions for simultaneous enhancement of humoral and cell-mediated immunity".ENGINEERING IN LIFE SCIENCES (2020):11.
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