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Manipulation of pore structure during manufacture of agarose microspheres for bioseparation
Zhao, Lan1; Huang, Yongdong1; Zhu, Kai1; Miao, Zhuang1; Zhao, Jiazhang1; Che, Xiang Jing1,2; Hao, Dongxia1; Zhang, Rongyue3; Ma, Guanghui1,4
2020-09-23
Source PublicationENGINEERING IN LIFE SCIENCES
ISSN1618-0240
Pages10
AbstractAgarose microspheres with a controllable pore structure were manufactured by varying agarose types and crosslinking degrees. Various agarose could tailor the gel formation of microspheres matrix and thus affect the final pore structures. Small pores in microspheres could be fabricated by agarose with a higher molecular weight, which was demonstrated by the packed column with lower distribution coefficient (K-av) values measured by gel filtration chromatography. Further, higherK(av)values also demonstrated that more and larger pores were formed with increasing the crosslinking degree of agarose microspheres. Either using agarose with a high molecular weight or increasing the crosslinking degree would finally lead to the enhancement of the flow rate during flow performance of packed column as necessary for improving separation efficiency. This provides a foundation for high-resolution chromatography with a controllable separation range as beneficial for downstream process.
Keywordagarose microspheres agarose types crosslinking degree gel filtration chromatography pore structure
DOI10.1002/elsc.202000023
Language英语
WOS KeywordGEL-BOUND ENZYMES ; CHROMATOGRAPHY ; EMULSIFICATION ; ADSORPTION ; ELECTROPHORESIS ; DERIVATIVES ; DIFFUSION ; UNIFORM ; BEADS ; SIZE
Funding ProjectNational Key Research and Development Program of China[2018YFC0311101] ; Beijing Municipal Natural Science Foundation - Haidian Original Innovation Joint Fund of China[L192017] ; National Natural Science Foundation of China[21676275] ; Youth Top Talent Project of Organization Department of Beijing Municipal Committee[ZZB2019001]
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
Funding OrganizationNational Key Research and Development Program of China ; Beijing Municipal Natural Science Foundation - Haidian Original Innovation Joint Fund of China ; National Natural Science Foundation of China ; Youth Top Talent Project of Organization Department of Beijing Municipal Committee
WOS IDWOS:000572075400001
PublisherWILEY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/42232
Collection中国科学院过程工程研究所
Corresponding AuthorHuang, Yongdong; Ma, Guanghui
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Yanshan Univ, Coll Environm & Chem Engn, Qinhuangdao, Hebei, Peoples R China
3.Beijing Inst Petrochem Technol, Coll Chem Engn, Beijing, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Lan,Huang, Yongdong,Zhu, Kai,et al. Manipulation of pore structure during manufacture of agarose microspheres for bioseparation[J]. ENGINEERING IN LIFE SCIENCES,2020:10.
APA Zhao, Lan.,Huang, Yongdong.,Zhu, Kai.,Miao, Zhuang.,Zhao, Jiazhang.,...&Ma, Guanghui.(2020).Manipulation of pore structure during manufacture of agarose microspheres for bioseparation.ENGINEERING IN LIFE SCIENCES,10.
MLA Zhao, Lan,et al."Manipulation of pore structure during manufacture of agarose microspheres for bioseparation".ENGINEERING IN LIFE SCIENCES (2020):10.
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