Knowledge Management System Of Institute of process engineering,CAS
| Manipulation of pore structure during manufacture of agarose microspheres for bioseparation | |
| Zhao, Lan1; Huang, Yongdong1; Zhu, Kai1; Miao, Zhuang1; Zhao, Jiazhang1; Che, Xiang Jing1,2; Hao, Dongxia1; Zhang, Rongyue3; Ma, Guanghui1,4 | |
| 2020-09-23 | |
| Source Publication | ENGINEERING IN LIFE SCIENCES
 |
| ISSN | 1618-0240 |
| Pages | 10 |
| Abstract | Agarose microspheres with a controllable pore structure were manufactured by varying agarose types and crosslinking degrees. Various agarose could tailor the gel formation of microspheres matrix and thus affect the final pore structures. Small pores in microspheres could be fabricated by agarose with a higher molecular weight, which was demonstrated by the packed column with lower distribution coefficient (K-av) values measured by gel filtration chromatography. Further, higherK(av)values also demonstrated that more and larger pores were formed with increasing the crosslinking degree of agarose microspheres. Either using agarose with a high molecular weight or increasing the crosslinking degree would finally lead to the enhancement of the flow rate during flow performance of packed column as necessary for improving separation efficiency. This provides a foundation for high-resolution chromatography with a controllable separation range as beneficial for downstream process. |
| Keyword | agarose microspheres agarose types crosslinking degree gel filtration chromatography pore structure |
| DOI | 10.1002/elsc.202000023 |
| Language | 英语 |
| WOS Keyword | GEL-BOUND ENZYMES ; CHROMATOGRAPHY ; EMULSIFICATION ; ADSORPTION ; ELECTROPHORESIS ; DERIVATIVES ; DIFFUSION ; UNIFORM ; BEADS ; SIZE |
| Funding Project | National Key Research and Development Program of China[2018YFC0311101] ; Beijing Municipal Natural Science Foundation - Haidian Original Innovation Joint Fund of China[L192017] ; National Natural Science Foundation of China[21676275] ; Youth Top Talent Project of Organization Department of Beijing Municipal Committee[ZZB2019001] |
| WOS Research Area | Biotechnology & Applied Microbiology |
| WOS Subject | Biotechnology & Applied Microbiology |
| Funding Organization | National Key Research and Development Program of China ; Beijing Municipal Natural Science Foundation - Haidian Original Innovation Joint Fund of China ; National Natural Science Foundation of China ; Youth Top Talent Project of Organization Department of Beijing Municipal Committee |
| WOS ID | WOS:000572075400001 |
| Publisher | WILEY |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/42232 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Huang, Yongdong; Ma, Guanghui |
| Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Yanshan Univ, Coll Environm & Chem Engn, Qinhuangdao, Hebei, Peoples R China 3.Beijing Inst Petrochem Technol, Coll Chem Engn, Beijing, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Zhao, Lan,Huang, Yongdong,Zhu, Kai,et al. Manipulation of pore structure during manufacture of agarose microspheres for bioseparation[J]. ENGINEERING IN LIFE SCIENCES,2020:10. |
| APA | Zhao, Lan.,Huang, Yongdong.,Zhu, Kai.,Miao, Zhuang.,Zhao, Jiazhang.,...&Ma, Guanghui.(2020).Manipulation of pore structure during manufacture of agarose microspheres for bioseparation.ENGINEERING IN LIFE SCIENCES,10. |
| MLA | Zhao, Lan,et al."Manipulation of pore structure during manufacture of agarose microspheres for bioseparation".ENGINEERING IN LIFE SCIENCES (2020):10. |
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