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Design and Reconstruction of Regulatory Parts for Fast-frowing Vibrio natriegens Synthetic Biology
Wu, Fengli1,2; Chen, Wujiu1; Peng, Yanfeng1; Tu, Ran1; Lin, Yuping1; Xing, Jianmin2; Wang, Qinhong1
2020-09-18
Source PublicationACS SYNTHETIC BIOLOGY
ISSN2161-5063
Volume9Issue:9Pages:2399-2409
AbstractThe fast-growing Vibrio natriegens is an attractive robust chassis for diverse synthetic biology applications. However, V. natriegens lacks the suitable constitutive regulatory parts for precisely tuning the gene expression and, thus, recapitulating physiologically relevant changes in gene expression levels. In this study, we designed, constructed, and screened the synthetic regulatory parts by varying the promoter region and ribosome binding site element for V. natriegens with different transcriptional or translational strengths, respectively. The fluorescence intensities of the cells with different synthetic regulatory parts could distribute evenly over a wide range of 5 orders of magnitude. The selected synthetic regulatory parts had good stability in both nutrient-rich and minimal media. The precise combinatorial modulation of galP (GalP = galactose permease) and glk (Glk = glucokinase) from Escherichia coli by using three synthetic regulatory parts with different strengths was confirmed in a phosphoenolpyruvate:carbohydrate phosphotransferase system with inactive V. natriegens strain to alter the glucose transport. This work provides the simple, efficient, and standardized constitutive regulatory parts for V. natriegens synthetic biology.
KeywordVibrio natriegens synthetic regulatory parts combinatorial modulation glucose transport synthetic biology
DOI10.1021/acssynbio.0c00158
Language英语
WOS KeywordEXPRESSION ; SEQUENCE ; PROTEIN ; TRANSLATION ; BACTERIUM ; PROMOTERS ; GLUCOSE
Funding ProjectNational Natural Science Foundation of China[31700080] ; China Postdoctoral Science Foundation[2017M621082] ; Enterprise Postdoctoral Innovation Project of Tianjin[TJQYBSH2017006]
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
Funding OrganizationNational Natural Science Foundation of China ; China Postdoctoral Science Foundation ; Enterprise Postdoctoral Innovation Project of Tianjin
WOS IDWOS:000574922400019
PublisherAMER CHEMICAL SOC
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Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/42246
Collection中国科学院过程工程研究所
Corresponding AuthorWang, Qinhong
Affiliation1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, CAS Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China
2.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Wu, Fengli,Chen, Wujiu,Peng, Yanfeng,et al. Design and Reconstruction of Regulatory Parts for Fast-frowing Vibrio natriegens Synthetic Biology[J]. ACS SYNTHETIC BIOLOGY,2020,9(9):2399-2409.
APA Wu, Fengli.,Chen, Wujiu.,Peng, Yanfeng.,Tu, Ran.,Lin, Yuping.,...&Wang, Qinhong.(2020).Design and Reconstruction of Regulatory Parts for Fast-frowing Vibrio natriegens Synthetic Biology.ACS SYNTHETIC BIOLOGY,9(9),2399-2409.
MLA Wu, Fengli,et al."Design and Reconstruction of Regulatory Parts for Fast-frowing Vibrio natriegens Synthetic Biology".ACS SYNTHETIC BIOLOGY 9.9(2020):2399-2409.
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