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Rapid and simple detection ofBacillus cereusin milk by real-time competitive annealing mediated isothermal amplification
Bao, Haiyan1; Li, Wei2; Yue, Xiqing1; Wu, Junrui1; Qiao, Yu3; Peng, Qing3; Shi, Bo3; Du, Yuguang4; Chen, Xu1; Wu, Rina1
2020-10-21
Source PublicationANALYST
ISSN0003-2654
Volume145Issue:20Pages:6677-6682
AbstractBacillus cereus(B. cereus) is widespread in nature and considered an important foodborne pathogen, which can lead to emetic syndrome and diarrheal illness. Therefore, appropriate detection methods are needed to effectively monitor this pathogenic bacterium. Competitive annealing mediated isothermal amplification (CAMP) is a novel nucleic-acid-based detection technology that amplifies DNA with high sensitivity and specificity under isothermal conditions. The aim of this study was to develop a real-time CAMP assay for the rapid and simple detection ofB. cereusin milk. In this system, a pair of primers was designed to specifically target theentFMgene ofB. cereus. Compared with the conventional PCR method, the CAMP assay has higher sensitivity, the same specificity and shorter detection time. The detection limits of the CAMP assay for pure bacterial cultures and artificially contaminated milk samples were all 59 CFU mL(-1). And this detection method showed a wide linear range (from 5.9 x 10(5)to 59 CFU mL(-1)) and satisfactory recovery values (from 75.76% to 106.78%). These results indicate that the developed CAMP assay is a potentially useful method for the detection ofB. cereusin milk.
DOI10.1039/d0an00965b
Language英语
WOS KeywordEMETIC BACILLUS-CEREUS ; ENTEROTOXIN GENES ; PROPIDIUM MONOAZIDE ; FOOD ; PCR ; STRAINS ; DIFFERENTIATION ; QUANTIFICATION ; ELISA ; ASSAY
Funding ProjectNational Key R D Project[2018YFD0501403] ; Youth Project of the Education Department of Liaoning Province, China[LSNQN201911]
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
Funding OrganizationNational Key R D Project ; Youth Project of the Education Department of Liaoning Province, China
WOS IDWOS:000576736000027
PublisherROYAL SOC CHEMISTRY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/42331
Collection中国科学院过程工程研究所
Corresponding AuthorChen, Xu; Wu, Rina
Affiliation1.Shenyang Agr Univ, Food Sci Coll, Shenyang 110866, Peoples R China
2.Shenyang Agr Univ, Dept Publicat, Shenyang 110866, Peoples R China
3.Chinese Acad Agr Sci, Feed Res Inst, Beijing 10008, Peoples R China
4.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Bao, Haiyan,Li, Wei,Yue, Xiqing,et al. Rapid and simple detection ofBacillus cereusin milk by real-time competitive annealing mediated isothermal amplification[J]. ANALYST,2020,145(20):6677-6682.
APA Bao, Haiyan.,Li, Wei.,Yue, Xiqing.,Wu, Junrui.,Qiao, Yu.,...&Wu, Rina.(2020).Rapid and simple detection ofBacillus cereusin milk by real-time competitive annealing mediated isothermal amplification.ANALYST,145(20),6677-6682.
MLA Bao, Haiyan,et al."Rapid and simple detection ofBacillus cereusin milk by real-time competitive annealing mediated isothermal amplification".ANALYST 145.20(2020):6677-6682.
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