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| Recovery of human serum albumin by dual-mode chromatography from the waste stream of Cohn fraction V supernatant | |
| Xiang, Jie1,2,3; Zhang, Songping4; Zhang, Guifeng4; Li, Xiunan4; Zhang, Chun1,2,3; Luo, Jian4; Yu, Rong1,2,3; Su, Zhiguo4 | |
| 2020-10-25 | |
| Source Publication | JOURNAL OF CHROMATOGRAPHY A
 |
| ISSN | 0021-9673 |
| Volume | 1630Pages:9 |
| Abstract | Plasma fractionation industry is by far the largest protein pharmaceutical provider, but there are still some plasma components in its industrial waste liquid that have not been utilized. This study aimed to develop a simple and efficient method for plasma protein recovery from Cohn fraction V supernatant (FVS), an effluent containing about 40% ethanol. A new affinity chromatography medium was synthesized with a fatty acid ligand. When the medium was applied to recovery of human serum albumin (HSA) from FVS at physiological pH7.4, the process was unsuccessful due to substantial decrease in capacity in the presence of high ethanol concentration. Nevertheless, change of pH from 7.4 to 4.2 emerged an improved adsorption capacity. The carboxyl group of the ligand began to act as cationic ion exchange role. Both HSA and alpha 2HS-glycoprotein were adsorbed by the column, but alpha 2HS-glycoprotein could be eluted by increasing pH from 4.2 to 7.4, while HSA was retained by the column and could only be eluted by addition of fatty acid. Therefore, the adsorption of albumin under pH 4.2 is charge-induced affinity adsorption, not simple ion exchange. The so-called dual-mode adsorption depends not only on the chromatographic medium but also on the separated object and environment. HPSEC showed that the purity of recovered HSA was greater than 98%. Circular dichroism and fluorescence spectra were consistent with that of the commercial product. Furthermore, the measurement by isothermal titration calorimetry showed that the separated HSA still maintained the binding activities with the ligands of warfarin and naproxen. It is therefore possible to directly recover high-purity and high-quality human serum albumin from the effluent of plasma fractionation industry by one-step chromatography. (C) 2020 Published by Elsevier B.V. |
| Keyword | Cohn fraction V supernatant Dual-mode chromatography Ion exchange Affinity Ethanol-water solution |
| DOI | 10.1016/j.chroma.2020.461451 |
| Language | 英语 |
| WOS Keyword | LARGE-SCALE PRODUCTION ; HUMAN SYNOVIAL FLUID ; PROTEINS ; BINDING ; PURIFICATION ; LIGAND ; SEPARATION |
| Funding Project | National Key R&D Program of China[2016YFD050080 0] ; National Key R&D Program of China[2016YFD0500809] ; National Natural Science Foundation of China[21821005] ; National Natural Science Foundation of China[81773623] |
| WOS Research Area | Biochemistry & Molecular Biology ; Chemistry |
| WOS Subject | Biochemical Research Methods ; Chemistry, Analytical |
| Funding Organization | National Key R&D Program of China ; National Natural Science Foundation of China |
| WOS ID | WOS:000576788800019 |
| Publisher | ELSEVIER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/42358 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Luo, Jian; Yu, Rong; Su, Zhiguo |
| Affiliation | 1.Sichuan Univ, West China Sch Pharm, Dept Biopharmaceut, Chengdu 610041, Peoples R China 2.Sichuan Univ, Key Lab Drug Targeting & Drug Delivery Syst, Educ Minist, Sichuan Engn Lab Plant Sourced Drug, Chengdu 610041, Peoples R China 3.Sichuan Univ, Sichuan Res Ctr Drug Precis Ind Technol, West China Sch Pharm, Chengdu 610041, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| Recommended Citation GB/T 7714 | Xiang, Jie,Zhang, Songping,Zhang, Guifeng,et al. Recovery of human serum albumin by dual-mode chromatography from the waste stream of Cohn fraction V supernatant[J]. JOURNAL OF CHROMATOGRAPHY A,2020,1630:9. |
| APA | Xiang, Jie.,Zhang, Songping.,Zhang, Guifeng.,Li, Xiunan.,Zhang, Chun.,...&Su, Zhiguo.(2020).Recovery of human serum albumin by dual-mode chromatography from the waste stream of Cohn fraction V supernatant.JOURNAL OF CHROMATOGRAPHY A,1630,9. |
| MLA | Xiang, Jie,et al."Recovery of human serum albumin by dual-mode chromatography from the waste stream of Cohn fraction V supernatant".JOURNAL OF CHROMATOGRAPHY A 1630(2020):9. |
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